与宿主红细胞膜相关的查巴迪疟原虫酸性磷酸蛋白的结构

Structure of a Plasmodium chabaudi acidic phosphoprotein that is associated with the host erythrocyte membrane.

作者信息

Deleersnijder W, Prasomsitti P, Tungpradubkul S, Hendrix D, Hamers-Casterman C, Hamers R

机构信息

Laboratorium Algemene Biologie, Instituut voor Moleculaire Biologie, Vrije Universiteit Brussel, Belgium.

出版信息

Mol Biochem Parasitol. 1992 Nov;56(1):59-68. doi: 10.1016/0166-6851(92)90154-c.

DOI:10.1016/0166-6851(92)90154-c

PMID:1475002

Abstract

We have characterized by molecular cloning and sequencing a Plasmodium chabaudi antigen that is associated with the membrane of the infected erythrocyte throughout the entire intraerythrocytic cycle. The protein (PcEMA1) has a predicted size of 50 kDa and contains a major tandem repeat array of 16 octapeptides that constitutes almost 30% of the protein. At its amino-terminus, PcEMA1 has a string of hydrophobic residues characteristic of a secreted protein, but does not contain a hydrophobic membrane-spanning segment. The antigen appears to reside on the cytoplasmic face of the erythrocytic membrane. PcEMA1 has a predicted pI of 4.4 and is a potential phosphoprotein.

摘要

我们通过分子克隆和测序鉴定了一种查巴迪疟原虫抗原，该抗原在整个红细胞内周期中均与受感染红细胞的膜相关。该蛋白（PcEMA1）预测大小为50 kDa，包含一个由16个八肽组成的主要串联重复序列，几乎占该蛋白的30%。在其氨基末端，PcEMA1有一串分泌蛋白特有的疏水残基，但不包含疏水跨膜片段。该抗原似乎位于红细胞膜的胞质面。PcEMA1预测的pI为4.4，是一种潜在的磷蛋白。