Favaloro J M, Kemp D J
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Mol Biochem Parasitol. 1994 Jul;66(1):39-47. doi: 10.1016/0166-6851(94)90034-5.
We have determined the complete genomic sequence of the Plasmodium chabaudi erythrocyte membrane antigen (PcEMA1) in 4 different parasite strains. The gene structure consisted of a short region encoding a signal sequence separated from the main coding region by an intervening sequence. The overall identity of the three P. chabaudi adami deduced protein sequences to their consensus was 100%, 99.8% and 88% for 556KA, DK and DS respectively, with a general pattern of increasing divergence from the N- to the C-terminus. The P. chabaudi chabaudi strain CB was 72% homologous to the P. chabaudi adami consensus sequence. A gene related to PcEMA1, designated PcEMA1-R, has been identified in the genome of P. chabaudi adami but not in P. chabaudi chabaudi. The partial sequence for this gene in P. chabaudi adami strain DS predicts that it could encode a truncated form of PcEMA1, but its status as a pseudogene or an independent, expressed gene has not been resolved.
我们已经确定了4种不同疟原虫菌株中查巴迪疟原虫红细胞膜抗原（PcEMA1）的完整基因组序列。该基因结构由一个编码信号序列的短区域组成，该区域通过一个间隔序列与主要编码区域分开。对于556KA、DK和DS这三种查巴迪疟原虫亚当斯株推导的蛋白质序列，它们与共识序列的总体一致性分别为100%、99.8%和88%，从N端到C端呈现出逐渐增加的差异模式。查巴迪疟原虫查巴迪株CB与查巴迪疟原虫亚当斯株共识序列的同源性为72%。在查巴迪疟原虫亚当斯株的基因组中鉴定出了一个与PcEMA1相关的基因，命名为PcEMA1-R，但在查巴迪疟原虫查巴迪株中未发现。查巴迪疟原虫亚当斯株DS中该基因的部分序列预测它可能编码PcEMA1的截短形式，但其作为假基因或独立的表达基因的状态尚未确定。
