Bettini E, Pollio G, Santagati S, Maggi A
Milan Molecular Pharmacology Laboratory, University of Milan, Italy.
Neuroendocrinology. 1992 Oct;56(4):502-8. doi: 10.1159/000126267.
A series of studies was done in order to fully characterize the estrogen receptor (ER) expressed in the hippocampus of adult female rat. The structural identity among the ER mRNAs expressed in the hippocampus, hypothalamus and uterus was established by polymerase chain reaction amplification of the ER cDNA. Subsequently, the ER of the hippocampus was proved to bind DNA and beta-estradiol with the same affinity as the hypothalamic receptor. Finally, it was demonstrated that systemic administration of beta-estradiol determines the nuclear increase of ER levels with a time course which appears to be almost superimposable in the hippocampus and hypothalamus. On the basis of the above-mentioned evidence, it is concluded that the ER expressed in the hippocampus is structurally and functionally indistinguishable from the receptor expressed in the other hormone target tissues.
为了全面表征成年雌性大鼠海马体中表达的雌激素受体（ER），进行了一系列研究。通过对ER cDNA进行聚合酶链反应扩增，确定了海马体、下丘脑和子宫中表达的ER mRNA之间的结构一致性。随后，证明海马体中的ER与下丘脑受体一样，以相同的亲和力结合DNA和β-雌二醇。最后，证明全身给予β-雌二醇可导致ER水平在细胞核中升高，其时间进程在海马体和下丘脑中几乎是重叠的。基于上述证据，得出结论：海马体中表达的ER在结构和功能上与其他激素靶组织中表达的受体没有区别。
