一种用于准确量化乳酸菌细菌素的快速比浊法微孔板生物测定法。
A rapid turbidometric microplate bioassay for accurate quantification of lactic acid bacteria bacteriocins.
作者信息
Turcotte Carl, Lacroix Christophe, Kheadr Ehab, Grignon Louis, Fliss Ismaïl
机构信息
Dairy Research Centre STELA, Pavillon Paul Comtois, Université Laval, Québec, PQ, Canada G1K7P4.
出版信息
Int J Food Microbiol. 2004 Feb 1;90(3):283-93. doi: 10.1016/s0168-1605(03)00315-5.
A 1 day turbidometric microplate bioassay (TMB) was developed for the rapid, accurate and precise quantification of lactic acid bacteria (LAB) bacteriocins (nisin Z and pediocin PA-1). Parameters such as the concentration of the indicator strains and the incubation time were optimized for each bacteriocin. A high correlation coefficient (r(2)=0.992+/-0.004) was obtained for the exponential regression in the nisin Z concentration range of 20-120 ng/ml with 1 x 10(7) CFU indicator strain (Pediococcus acidilactici UL5) and an incubation time of 3 h. Using these parameters, the detection limit was estimated at 80 ng/ml (3.2 IU/ml), compared to 300 ng/ml for the agar diffusion assay (ADA). High precision (<7%) and accuracy (10%) were obtained for all nisin Z concentrations tested. Similar results were obtained with pediocin PA-1 with r(2)=0.993+/-0.005, a precision (8.2%) and an accuracy lower than 15%.
开发了一种1天的比浊法微孔板生物测定法（TMB），用于快速、准确和精确地定量乳酸菌（LAB）细菌素（乳酸链球菌素Z和片球菌素PA-1）。针对每种细菌素，对指示菌株浓度和孵育时间等参数进行了优化。对于乳酸链球菌素Z，在浓度范围为20 - 120 ng/ml、指示菌株（嗜酸乳杆菌UL5）为1×10⁷ CFU且孵育时间为3小时的情况下，指数回归获得了较高的相关系数（r² = 0.992 ± 0.004）。使用这些参数，检测限估计为80 ng/ml（3.2 IU/ml），而琼脂扩散法（ADA）的检测限为300 ng/ml。对于所有测试的乳酸链球菌素Z浓度，均获得了高精度（<7%）和准确度（10%）。片球菌素PA-1也得到了类似的结果，r² = 0.993 ± 0.005，精密度为8.2%，准确度低于15%。
Zotero 插件