厌氧微生物学与生物技术中生长和生产力的量化方法。
Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology.
作者信息
Mauerhofer Lisa-Maria, Pappenreiter Patricia, Paulik Christian, Seifert Arne H, Bernacchi Sébastien, Rittmann Simon K-M R
机构信息
Archaea Physiology & Biotechnology Group, Archaea Biology and Ecogenomics Division, Department of Ecogenomics and Systems Biology, Universität Wien, Althanstraße 14, 1090, Wien, Austria.
Institute for Chemical Technology of Organic Materials, Johannes Kepler University Linz, Linz, Austria.
出版信息
Folia Microbiol (Praha). 2019 May;64(3):321-360. doi: 10.1007/s12223-018-0658-4. Epub 2018 Nov 16.
Anaerobic microorganisms (anaerobes) possess a fascinating metabolic versatility. This characteristic makes anaerobes interesting candidates for physiological studies and utilizable as microbial cell factories. To investigate the physiological characteristics of an anaerobic microbial population, yield, productivity, specific growth rate, biomass production, substrate uptake, and product formation are regarded as essential variables. The determination of those variables in distinct cultivation systems may be achieved by using different techniques for sampling, measuring of growth, substrate uptake, and product formation kinetics. In this review, a comprehensive overview of methods is presented, and the applicability is discussed in the frame of anaerobic microbiology and biotechnology.
厌氧微生物具有令人着迷的代谢多样性。这一特性使厌氧微生物成为生理学研究的有趣对象，并可作为微生物细胞工厂加以利用。为了研究厌氧微生物群体的生理特性，产量、生产率、比生长速率、生物量生产、底物摄取和产物形成被视为关键变量。在不同的培养系统中测定这些变量，可以通过使用不同的采样技术、生长测量技术、底物摄取和产物形成动力学测量技术来实现。在这篇综述中，对相关方法进行了全面概述，并在厌氧微生物学和生物技术的框架内讨论了其适用性。
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