[酶在不同结构离子交换树脂上的吸附作用]

[Sorption of enzymes on ion exchange resins of various structure].

作者信息

Rudometova N V, Efimova T B, Glazova N V, Dmitrenko L V

出版信息

Prikl Biokhim Mikrobiol. 1992 Sep-Oct;28(5):694-7.

Abstract

The isothermic adsorption of microbial and animal enzymes on carboxyl and sulpha-cation exchange resins was studied. The adsorption isotherms are curves with a maximum. The adsorption of alpha-amylase was studied in the presence of organic solvents. It was found that organic solvents influenced the isothermic adsorption of alpha-amylase, which is associated with changes in the interactions between protein molecules in solution. The adsorption system was in equilibrium in all the cases.

摘要

研究了微生物酶和动物酶在羧基及磺酸阳离子交换树脂上的等温吸附。吸附等温线为具有最大值的曲线。在有机溶剂存在的情况下研究了α-淀粉酶的吸附。发现有机溶剂影响α-淀粉酶的等温吸附，这与溶液中蛋白质分子间相互作用的变化有关。在所有情况下吸附体系均处于平衡状态。