van Tol E A, Verspaget H W, Peña A S, Lamers C B
Dept. of Gastroenterology and Hepatology, University Hospital, Leiden, The Netherlands.
Scand J Gastroenterol. 1992 Dec;27(12):999-1005. doi: 10.3109/00365529209028129.
Non-major histocompatibility complex-restricted cytotoxicity or natural killer (NK) activity could be detected in all intestinal lamina propria mononuclear cell preparations of histologically normal mucosa from 57 patients with gastrointestinal disease. Similar levels of NK activity were detected among the different disease groups. Within the inflammatory bowel disease patient group, however, Crohn's disease patients showed a threefold higher level of NK activity than detected in ulcerative colitis patients. Cytotoxicity levels in Crohn's disease patients were also higher than in the control carcinoma patients, whereas ulcerative colitis patients had considerably lower cytotoxicity levels than the carcinoma patients. Thus, unaffected normal inflammatory bowel disease mucosa conceals alterations in NK activity which might occur before the inflammation. The colon adenocarcinoma cell line Caco-2 was found to be a representative target for detecting individual differences in NK activity of lamina propria mononuclear cells compared with standard K-562 targets. The latter can be of relevance when studying mucosal immunoregulatory mechanisms in intestinal disease.
在57例胃肠道疾病患者组织学正常黏膜的所有肠固有层单核细胞制剂中，均可检测到非主要组织相容性复合体限制的细胞毒性或自然杀伤（NK）活性。在不同疾病组中检测到相似水平的NK活性。然而，在炎症性肠病患者组中，克罗恩病患者的NK活性水平比溃疡性结肠炎患者高出三倍。克罗恩病患者的细胞毒性水平也高于对照癌患者，而溃疡性结肠炎患者的细胞毒性水平则明显低于癌患者。因此，未受影响的正常炎症性肠病黏膜掩盖了炎症之前可能发生的NK活性改变。与标准K-562靶细胞相比，结肠腺癌细胞系Caco-2被发现是检测固有层单核细胞NK活性个体差异的代表性靶细胞。在研究肠道疾病的黏膜免疫调节机制时，后者可能具有相关性。
