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[正常人及牛淋巴细胞细胞核在病理学中的光度学特征]

[The photometric characteristics of the nuclei of normal human and bovine lymphocytes and in pathology].

作者信息

Novikov V D, Iasakova N T, Lisichenko O V, Luk'ianova T V, Avdeenko V S, Belan I B

出版信息

Tsitologiia. 1992;34(7):47-52.

PMID:1475852
Abstract

Chromatin structure has been studied with a method of optico-structural machine analysis for interphase nuclei of peripheral blood lymphocytes in healthy donors, a girl with the Shereshevskiĭ-Turner syndrome (45, XO) and her parents, and for peripheral blood lymphocytes of a cow, Bos taurus L., in the last trimester on the background of preeclamptic syndrome and with normal cow pregnancy of compared terms. A significant change was revealed both in human heteroploidy and in developing preeclamptic syndrome in animals as concerns such indexes as the nucleus area, integral optical density, the amount and area of condensed, decondensed chromatin. The profile of histograms, according to the given parameters, is distinctly changing. The analysis based on constructing a two-measured field in the given coordinate system showed changes in the lymphatic population structure with the increase in the rate of "heavy", enriched DNA cells.

摘要

采用光学结构机器分析方法,对健康供体外周血淋巴细胞的间期核、一名患有舍雷舍夫斯基-特纳综合征(45,XO)的女孩及其父母的外周血淋巴细胞,以及处于先兆子痫综合征晚期和正常妊娠同期的母牛(Bos taurus L.)外周血淋巴细胞的染色质结构进行了研究。结果发现,在人类异倍体以及动物先兆子痫综合征的发展过程中,细胞核面积、积分光密度、凝聚和去凝聚染色质的数量及面积等指标均发生了显著变化。根据给定参数绘制的直方图轮廓也明显改变。在给定坐标系中构建二维测量场的分析表明,随着“重”的、富含DNA细胞比例的增加,淋巴细胞群体结构发生了变化。

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