Yuan Ling, Michels E, De Meester L
Laboratory of Ecology and Aquaculture, Katholieke Universiteit Leuven, Belgium.
J Environ Sci (China). 2003 Nov;15(6):841-7.
In order to develop a sound biotechnique for monitoring water quality that builds on the previous experiments carried out in our laboratory, a specific D. magna clone C1 242 was used to study the effects of pollutants on phototactic behavior. In all experiments, the animals showed a stable and repeatable phototactic index approximated 0.2 in the presence and 0.4 in the absence of fish kairomones, which decreased significantly in response to pollutants. There existed no pollutant x fish kairomone interaction, indicating the changes in phototactic behavior of animals imposed by pollutants were independent of the presence of fish kairomones. The detection limits for changes in phototactic behavior of D. magna clone C1 242 are 0.04 mg/L for copper, 0.02 mg/L for cadmium, and 0.80 mg/L for PCP, respectively, quite lower than LC50 (48 h). The changes in phototactic behavior in presence to pollutants occurred quickly (3 h) compared to the period over whole acute toxicity tests. Therefore, D. magna clone C1 242 could be potentially used to monitor water quality. Moreover, the phototactic behavior did not decrease further in the pollutant mixtures employed in our experiments compared to individual pollutants, except in the Cd-PCP treatment. This fact suggests that the formation of water quality criteria must be based upon pollutant mixture tests.
为了在我们实验室之前进行的实验基础上开发一种完善的水质监测生物技术，使用特定的大型溞克隆C1 242来研究污染物对趋光行为的影响。在所有实验中，动物在有鱼信息素时趋光指数稳定且可重复，约为0.2，无鱼信息素时为0.4，而在接触污染物后趋光指数显著下降。不存在污染物×鱼信息素的相互作用，这表明污染物引起的动物趋光行为变化与鱼信息素的存在无关。大型溞克隆C1 242趋光行为变化的检测限分别为：铜0.04 mg/L、镉0.02 mg/L、五氯苯酚0.80 mg/L，远低于半数致死浓度（48小时）。与整个急性毒性试验的时间相比，接触污染物后趋光行为的变化发生得很快（3小时）。因此，大型溞克隆C1 242有可能用于监测水质。此外，与单独污染物相比，除了镉 - 五氯苯酚处理外，我们实验中使用的污染物混合物并未使趋光行为进一步下降。这一事实表明，水质标准的制定必须基于污染物混合物试验。
