Selga Ariadna, Sort Xavier, Bobet Raül, Torres Josep Lluís
Institute for Chemical and Environmental Research (IIQAB-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain.
J Agric Food Chem. 2004 Feb 11;52(3):467-73. doi: 10.1021/jf035048l.
Antioxidant thio-conjugates were obtained from white grape pomace by a clean and efficient one pot extraction and depolymerization method using water and cysteamine hydrochloride. To evaluate the potential of grape pomaces of different origins as sources of proanthocyanidins and conjugates, we conducted varietal comparative studies of polyphenol content, antioxidant power, and procyanidin composition. Xarel.lo proved to be the richest source of polyphenols. The total conversion into the conjugates was as high as 8 g/kg of Xarel.lo grape pomace, with a 50-fold excess of cysteamine, and 3 g/kg, with a 5-fold excess of cysteamine. After purification by preparative cation exchange and reversed phase high-performance liquid chromatography, 17 g of 63% pure 4beta-(2-aminoethylthio)epicatechin (acetate salt) was obtained from 35 kg of moist pomace. The procedure described here will make the antioxidant thio-derivatives efficiently available directly from raw plant byproducts such as grape pomace.
通过使用水和盐酸半胱胺的清洁高效一锅法提取和解聚方法，从白葡萄皮渣中获得了抗氧化硫代共轭物。为了评估不同来源的葡萄皮渣作为原花青素和共轭物来源的潜力，我们对多酚含量、抗氧化能力和原花青素组成进行了品种比较研究。沙雷洛被证明是最丰富的多酚来源。在半胱胺过量50倍的情况下，转化为共轭物的总量高达8克/千克沙雷洛葡萄皮渣，在半胱胺过量5倍的情况下为3克/千克。通过制备型阳离子交换和反相高效液相色谱法纯化后，从35千克湿皮渣中获得了17克纯度为63%的4β-(2-氨基乙硫基)表儿茶素（醋酸盐）。这里描述的方法将使抗氧化硫代衍生物能够直接从葡萄皮渣等植物原料副产物中高效获得。
