Amano S, Sawa M, Ishii Y
Department of Ophthalmology, University of Tokyo School of Medicine, Bunkyo-ku, Japan.
Nippon Ganka Gakkai Zasshi. 1992 Nov;96(11):1366-72.
A model for keratoepithelioplasty (KEP) was developed using the Lewis rat, and histological studies were performed using the model. The entire corneal epithelium was removed using a spatula and a 1.5-mm-width of the conjunctiva including the limbus was excised. An oval corneal lamellar graft (3 x 1.5 mm) with an intact epithelium taken from another Lewis rat was transplanted on the denuded limbus. Biomicroscopic observation showed significantly less vascular invasion in the part of the cornea adjacent to the lenticule than in other part of cornea, and clear cornea was maintained in the cornea adjacent to the lenticule. Histologically only few vessels were recognized in the lenticule, and the epithelial cells on the lenticule showed histological characteristics of corneal epithelium. These results indicate that surgical function of KEP can be obtained because the lenticules keep distance between corneal epithelium and conjunctival vessels. And it is also confirmed that this model is useful in research on the pathophysiological mechanism of KEP.
利用Lewis大鼠建立了角膜上皮成形术（KEP）模型，并使用该模型进行了组织学研究。用刮匙去除整个角膜上皮，并切除包括角膜缘在内的1.5毫米宽的结膜。将取自另一只Lewis大鼠的带有完整上皮的椭圆形角膜板层移植物（3×1.5毫米）移植到裸露的角膜缘上。生物显微镜观察显示，与角膜其他部位相比，靠近晶状体的角膜部分血管侵入明显较少，且靠近晶状体的角膜保持透明。组织学上，在晶状体中仅发现少量血管，晶状体上的上皮细胞显示出角膜上皮的组织学特征。这些结果表明，由于晶状体保持了角膜上皮与结膜血管之间的距离，因此可以获得KEP的手术效果。并且还证实该模型在KEP病理生理机制的研究中是有用的。
