Cho Sayeon, Park Sung Goo, Lee Do Hee, Park Byoung Chul
Laboratory of Proteome Analysis, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Daejeon 305-600, South Korea.
J Biochem Mol Biol. 2004 Jan 31;37(1):45-52. doi: 10.5483/bmbrep.2004.37.1.045.
The goal of interaction proteomics that studies the protein-protein interactions of all expressed proteins is to understand biological processes that are strictly regulated by these interactions. The availability of entire genome sequences of many organisms and high-throughput analysis tools has led scientists to study the entire proteome (Pandey and Mann, 2000). There are various high-throughput methods for detecting protein interactions such as yeast two-hybrid approach and mass spectrometry to produce vast amounts of data that can be utilized to decipher protein functions in complicated biological networks. In this review, we discuss recent developments in analytical methods for large-scale protein interactions and the future direction of interaction proteomics.
研究所有表达蛋白质的蛋白质-蛋白质相互作用的相互作用蛋白质组学的目标是了解受这些相互作用严格调控的生物过程。许多生物体全基因组序列的可得性以及高通量分析工具促使科学家们去研究整个蛋白质组（潘迪和曼，2000年）。有多种用于检测蛋白质相互作用的高通量方法，如酵母双杂交方法和质谱分析法，以产生大量可用于解读复杂生物网络中蛋白质功能的数据。在本综述中，我们讨论了大规模蛋白质相互作用分析方法的最新进展以及相互作用蛋白质组学的未来方向。
