Xiao Zhi-jian
State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
Zhonghua Xue Ye Xue Za Zhi. 2003 Dec;24(12):621-3.
To investigate the relationship between topoisomerase II inhibitors and t(8;21) chromosomal translocation.
The rearrangements of AML1 and ETO genes were detected by Southern Blot and the AML1-ETO fusion gene by nested RT-PCR combined with sequencing in K562 cells treated with etoposide (Vp16) and doxorubicin (DOX).
The rearrangements of AML1 gene were detectable after DOX treatment at concentrations of 10, 50 and 100 micro mol/L for 16 h, AML1-ETO fusion gene appeared after 50 micro mol/L DOX treatment for 48 h.
Induction of AML1 gene rearrangement and fusion by topoisomerase II inhibitors, represents one of the molecular mechanisms of t(8;21) chromosomal translocation.
研究拓扑异构酶II抑制剂与t(8;21)染色体易位之间的关系。
采用Southern印迹法检测阿霉素（Vp16）和柔红霉素（DOX）处理的K562细胞中AML1和ETO基因的重排，并通过巢式逆转录聚合酶链反应（RT-PCR）结合测序检测AML1-ETO融合基因。
在浓度为10、50和100微摩尔/升的DOX处理16小时后可检测到AML1基因重排，在50微摩尔/升DOX处理48小时后出现AML1-ETO融合基因。
拓扑异构酶II抑制剂诱导AML1基因重排和融合，是t(8;21)染色体易位的分子机制之一。
