[贵州省莱姆病螺旋体核糖体分型研究]
[Study on ribotyping of Lyme borreliosis spirochete in Guizhou province].
作者信息
Wang Ding-ming, Hao Qin, Cai Xing-he, Wan Kang-lin, Wang Zhao-xiao, Chen Jian
机构信息
Guizhou Center for Disease Control and Prevention, Guiyang 550004, China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Dec;24(12):1129-31.
OBJECTIVE
To define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investigation of 21 Borrelia burgdorferi sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy.
METHODS
Polymerase chain reaction (PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer, and amplified products were analyzed by restriction fragment length polymorphism (RFLP) and nucleotide sequencing.
RESULTS
There were two genospecies in the strains: 20 strains belong to Borrelia valaisiana, 1 strain is Borelia sp.
CONCLUSION
Borrelia valaisiana was the main genotype in Guizhou agricultural areas. The harmness of B. valaisiana to human being has been confirmed. In order to efficiently prevent the harmness of agent to the people in Guizhou agriculture areas, we should study the risk further.
目的
通过对21株莱姆病螺旋体狭义伯氏疏螺旋体进行分子流行病学调查，明确贵州农业地区的主要基因型，为制定预防策略提供科学依据。
方法
采用聚合酶链反应（PCR）技术扩增23S（rrl）-5S（rrf）基因间隔区，扩增产物经限制性片段长度多态性（RFLP）分析及核苷酸测序。
结果
菌株中有两个基因种：20株属于瓦莱州疏螺旋体，1株为疏螺旋体属。
结论
瓦莱州疏螺旋体是贵州农业地区的主要基因型。瓦莱州疏螺旋体对人类的危害性已得到证实。为有效预防病原体对贵州农业地区人群的危害，应进一步研究其风险。
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