木霉菌（Trichoderma virens）中丝氨酸蛋白酶编码基因tvsp1的功能分析。

Functional analysis of tvsp1, a serine protease-encoding gene in the biocontrol agent Trichoderma virens.

作者信息

Pozo María J, Baek Jong-Min, García Juan M, Kenerley Charles M

机构信息

Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA.

出版信息

Fungal Genet Biol. 2004 Mar;41(3):336-48. doi: 10.1016/j.fgb.2003.11.002.

DOI:10.1016/j.fgb.2003.11.002

PMID:14761794

Abstract

Serine proteases are highly conserved among fungi and considered to play a key role in different aspects of fungal biology. These proteases can be involved in development and have been related to pathogenesis or biocontrol processes. A gene (tvsp1) encoding an extracellular serine protease was cloned from Trichoderma virens, a biocontrol agent effective against soilborne fungal pathogens. The gene was expressed in Escherichia coli and a polyclonal antibody was raised against the recombinant protein. The expression pattern of tvsp1 was determined and its physiological role was addressed by mutational analysis. Strains of T. virens in which tvsp1 was deleted (PKO) or constitutively overexpressed (POE) were not affected in growth rate, conidiation, extracellular protein accumulation, antibiotic profiles nor in their ability to induce phytoalexins in cotton seedlings. Tvsp1 overexpression, however, significantly increased the ability of some strains to protect cotton seedlings against Rhizoctonia solani. Our data show that Tvsp1 is not necessary for the normal growth or development of T. virens, but plays a role in the biocontrol process.

摘要

丝氨酸蛋白酶在真菌中高度保守，被认为在真菌生物学的不同方面发挥关键作用。这些蛋白酶可参与真菌发育，并与致病过程或生物防治过程有关。从绿色木霉（一种对土壤传播的真菌病原体有效的生物防治剂）中克隆了一个编码细胞外丝氨酸蛋白酶的基因（tvsp1）。该基因在大肠杆菌中表达，并制备了针对重组蛋白的多克隆抗体。通过突变分析确定了tvsp1的表达模式并探讨了其生理作用。tvsp1被缺失（PKO）或组成型过表达（POE）的绿色木霉菌株在生长速率、分生孢子形成、细胞外蛋白积累、抗生素谱以及诱导棉花幼苗中植保素的能力方面均未受到影响。然而，tvsp1的过表达显著提高了一些菌株保护棉花幼苗免受立枯丝核菌侵害的能力。我们的数据表明，Tvsp1对于绿色木霉的正常生长或发育不是必需的，但在生物防治过程中发挥作用。