Tamiya E, Karube I
Research Center for Advanced Science and Technology, University of Tokyo, Japan.
Ann N Y Acad Sci. 1992 Nov 30;672:272-7. doi: 10.1111/j.1749-6632.1992.tb32690.x.
Carbon fiber electrodes are used to construct ultramicrobiosensors with 7-15 microns diameter. Electrochemical operations for preelectrolysis and measuring were examined for sensitive determination of hydrogen peroxide. Determination limit was 0.1 microM of hydrogen peroxide. Reproducible determination of hydrogen peroxide is possible even in samples containing albumin protein. A micro-acetylcholine sensor was fabricated by immobilizing acetylcholine esterase and choline oxidase on the carbon fiber by entrapment with PVA-SbQ. This sensor gave a linear calibration plot for the range from 0.1 to 1.0 mM with a linear correlation coefficient of 0.9842. A micro-glutamate sensor consisted of a platinized carbon fiber disk electrode modified with immobilized glutamate oxidase membrane. This sensor gave a linear calibration for the range 2 microM to 1.2 mM. Release of glutamate in the cerebellar cortex was detected after potassium stimulation.
碳纤维电极用于构建直径为7 - 15微米的超微生物传感器。研究了用于预电解和测量的电化学操作，以灵敏测定过氧化氢。过氧化氢的测定限为0.1微摩尔。即使在含有白蛋白的样品中，也能够重现性地测定过氧化氢。通过用聚乙烯醇 - 锑醌包埋将乙酰胆碱酯酶和胆碱氧化酶固定在碳纤维上，制备了一种微型乙酰胆碱传感器。该传感器在0.1至1.0毫摩尔范围内给出线性校准曲线，线性相关系数为0.9842。一种微型谷氨酸传感器由用固定化谷氨酸氧化酶膜修饰的镀铂碳纤维圆盘电极组成。该传感器在2微摩尔至1.2毫摩尔范围内给出线性校准。在钾刺激后检测到小脑皮质中谷氨酸的释放。
