Peng Xi, Chen Rong-Chun, Wang Pei, You Zhong-Yi, Wang Shi-Liang
Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2004 Feb;16(2):93-6.
To investigate the effects of enteral supplementation with glutamine on mitochondria respiratory function of intestinal epithelium in burned rats.
Wistar rats inflicted with 30% total body surface area (TBSA) full thickness thermal injury were randomly divided into three groups, i.e. burn with enteral nutrition (EN), burn with glutamine treatment (GLN), and normal control (C) groups. Burned rats were infused 732.2 kJ.kg-1.d-1 solution for intravenous nutrition and oral administration, in which the supply energy ratio of glucose, fat and protein was 55:30:15 respectively, glucose was 15.3% and the proportion of calorie to nitrogen was 183:1. The following indices including respiratory control rate (RCR), oxygen extraction (Oext), P/O ratio and intestine mucosal blood flow (IMBF) were measured on postburn days 1, 3, 5, 7, 10.
After burn injury, the RCR, Oext, P/O ratio, and IMBF were significant decreased in both EN and GLN groups, but all above indices were markedly increased in GLN group compared to those in EN group.
After burn injury, the IMBF and Oext were declined, resulting in mitochondria respiratory oxidative dysfunction and phosphorylation discoupling in intestinal epithelium. GLN supplementation appears to be beneficial to improving IMBF, increasing Oext, abating the extent of mitochondria respiration dysfunction, and promoting oxidative phosphorylation.
探讨肠内补充谷氨酰胺对烧伤大鼠肠上皮线粒体呼吸功能的影响。
将30%总体表面积（TBSA）全层热烧伤的Wistar大鼠随机分为三组，即烧伤肠内营养（EN）组、烧伤谷氨酰胺治疗（GLN）组和正常对照（C）组。给烧伤大鼠静脉输注732.2 kJ·kg-1·d-1溶液进行肠内营养，其中葡萄糖、脂肪和蛋白质供能比分别为55:30:15，葡萄糖含量为15.3%，热氮比为183:1。于烧伤后第1、3、5、7、10天测量以下指标，包括呼吸控制率（RCR）、氧摄取率（Oext）、P/O比值和肠黏膜血流量（IMBF）。
烧伤后，EN组和GLN组的RCR、Oext、P/O比值和IMBF均显著降低，但与EN组相比，GLN组上述指标均明显升高。
烧伤后，IMBF和Oext降低，导致肠上皮线粒体呼吸氧化功能障碍和磷酸化解偶联。补充谷氨酰胺似乎有利于改善IMBF、增加Oext、减轻线粒体呼吸功能障碍程度并促进氧化磷酸化。
