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本文引用的文献

1
[Diagnosis of imported malaria with multiplex PCR on LighCycler apparatus].
Pathol Biol (Paris). 2003 Feb;51(1):44-6. doi: 10.1016/s0369-8114(02)00319-x.
2
Identification of Plasmodium falciparum, P. vivax, P. ovale and P. malariae and detection of mixed infection in patients with imported malaria in Italy.意大利输入性疟疾患者中恶性疟原虫、间日疟原虫、卵形疟原虫和三日疟原虫的鉴定及混合感染检测
New Microbiol. 2003 Jan;26(1):91-100.
3
New strategies for the diagnosis and screening of malaria.疟疾诊断与筛查的新策略
Int J Hematol. 2002 Aug;76 Suppl 1:291-3. doi: 10.1007/BF03165265.
4
How useful is PCR in the diagnosis of malaria?聚合酶链反应（PCR）在疟疾诊断中作用有多大？
Trends Parasitol. 2002 Sep;18(9):395-8. doi: 10.1016/s1471-4922(02)02348-6.
5
Plasmodium vivax: parasitemia determination by real-time quantitative PCR in Aotus monkeys.间日疟原虫：通过实时定量PCR测定夜猴体内的疟原虫血症
Exp Parasitol. 2002 Feb;100(2):131-4. doi: 10.1016/S0014-4894(02)00010-3.
6
Transcripts of developmentally regulated Plasmodium falciparum genes quantified by real-time RT-PCR.通过实时逆转录聚合酶链反应定量分析的恶性疟原虫发育调控基因转录本。
Nucleic Acids Res. 2002 May 15;30(10):2224-31. doi: 10.1093/nar/30.10.2224.
7
Detection of Plasmodium falciparum malaria parasites in vivo by real-time quantitative PCR.通过实时定量PCR在体内检测恶性疟原虫疟原虫
Mol Biochem Parasitol. 2001 Dec;118(2):247-51. doi: 10.1016/s0166-6851(01)00379-6.
8
Determining liver stage parasite burden by real time quantitative PCR as a method for evaluating pre-erythrocytic malaria vaccine efficacy.通过实时定量PCR确定肝脏期寄生虫负荷作为评估红细胞前期疟疾疫苗效力的一种方法。
Mol Biochem Parasitol. 2001 Dec;118(2):233-45. doi: 10.1016/s0166-6851(01)00372-3.
9
Detection of malaria liver-stages in mice infected through the bite of a single Anopheles mosquito using a highly sensitive real-time PCR.使用高灵敏度实时聚合酶链反应检测通过单只按蚊叮咬感染的小鼠体内的疟原虫肝期。
Int J Parasitol. 2001 Nov;31(13):1499-502. doi: 10.1016/s0020-7519(01)00265-x.
10
Comparison of the ParaSight-F test and the ICT Malaria Pf test with the polymerase chain reaction for the diagnosis of Plasmodium falciparum malaria in travellers.ParaSight-F检测和ICT疟疾Pf检测与聚合酶链反应在诊断旅行者恶性疟原虫疟疾中的比较。
Trans R Soc Trop Med Hyg. 1998 Mar-Apr;92(2):166-9. doi: 10.1016/s0035-9203(98)90730-1.

用于疟疾诊断的RealArt疟疾实时荧光定量PCR检测方法的评估

Evaluation of the RealArt Malaria LC real-time PCR assay for malaria diagnosis.

作者信息

Farcas Gabriella A, Zhong Kathleen J Y, Mazzulli Tony, Kain Kevin C

机构信息

Institute of Medical Science, Department of Medicine, McLaughlin Center for Molecular Medicine, University of Toronto, Ontario, Canada.

出版信息

J Clin Microbiol. 2004 Feb;42(2):636-8. doi: 10.1128/JCM.42.2.636-638.2004.

DOI:10.1128/JCM.42.2.636-638.2004
PMID:14766829
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC344507/
Abstract

PCR-based methods have advantages over traditional microscopic methods for the diagnosis of malaria, especially in cases of low parasitemia and mixed infections. However, current PCR-based assays are often labor-intensive and not readily quantifiable and have the potential for contamination due to a requirement for postamplification sample handling. Real-time PCR can address these limitations. This study evaluated the performance characteristics of a commercial malaria real-time PCR assay (RealArt Malaria LC Assay; Artus GmbH, Hamburg, Germany) on the LightCycler platform for the detection of malaria parasites in 259 febrile returned travelers. Compared to nested PCR as the reference standard, the real-time assay had a sensitivity of 99.5%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 99.6% for the detection of malaria. Our results indicate that the RealArt assay is a rapid (<45 min), sensitive, and specific method for the detection of malaria in returned travelers.

摘要

基于聚合酶链反应（PCR）的方法在疟疾诊断方面比传统显微镜检查方法具有优势，尤其是在低疟原虫血症和混合感染的情况下。然而，目前基于PCR的检测方法通常劳动强度大，不易定量，并且由于需要对扩增后样本进行处理，存在污染的可能性。实时PCR可以解决这些局限性。本研究评估了一种用于疟疾的商业实时PCR检测方法（RealArt疟疾轻链检测法；德国汉堡阿图斯有限公司）在LightCycler平台上对259名发热回国旅行者疟原虫检测的性能特征。与作为参考标准的巢式PCR相比，该实时检测方法检测疟疾的灵敏度为99.5%，特异性为100%，阳性预测值为100%，阴性预测值为99.6%。我们的结果表明，RealArt检测法是一种快速（<45分钟）、灵敏且特异的检测回国旅行者疟疾的方法。