Banu Sayera, Gordon Stephen V, Palmer Si, Islam M Rizaul, Ahmed Shakeel, Alam Khan Mashrequl, Cole Stewart T, Brosch Roland
ICDDR,B: Centre for Health and Population Research, Dhaka-1000, Bangladesh.
J Clin Microbiol. 2004 Feb;42(2):674-82. doi: 10.1128/JCM.42.2.674-682.2004.
Genotypic analysis was performed on 48 Mycobacterium tuberculosis complex strains collected from a hospital in Dhaka city. Deletion analysis showed that the isolates were all M. tuberculosis; 13 of them were found to be of the "ancestral" type, while 35 were of the "modern" type, indicating that both endemic (ancestral type) and epidemic (modern type) strains cause tuberculosis in Bangladesh. Genotyping based on the spoligotype and variable-number tandem repeats (VNTR) of mycobacterial interspersed repetitive units (MIRU) was also done. A total of 34 strains (71%) were grouped by spoligotyping into nine different clusters; the largest comprised 15 isolates of the Beijing genotype, whereas the remaining eight clusters consisted of two to five isolates. MIRU-VNTR typing detected 32 different patterns among 44 tested strains, and the 15 Beijing strains were further discriminated by MIRU-VNTR typing (7 distinct patterns for the 15 isolates). These results indicate that MIRU-VNTR typing, along with spoligotyping and deletion analysis, can be used effectively for molecular epidemiological studies to determine ongoing transmission clusters; to our knowledge, this is the first report about the type of strains prevailing in Bangladesh.
对从达卡市一家医院收集的48株结核分枝杆菌复合群菌株进行了基因分型分析。缺失分析表明，这些分离株均为结核分枝杆菌；其中13株为“祖先型”，35株为“现代型”，这表明在孟加拉国，地方流行菌株（祖先型）和流行菌株（现代型）均可导致结核病。还基于分枝杆菌散布重复单元（MIRU）的间隔寡核苷酸分型（spoligotype）和可变数目串联重复序列（VNTR）进行了基因分型。共有34株菌株（71%）通过间隔寡核苷酸分型被归为9个不同的簇；最大的簇包含15株北京基因型分离株，其余8个簇由2至5株分离株组成。MIRU-VNTR分型在44株检测菌株中检测到32种不同模式，15株北京菌株通过MIRU-VNTR分型进一步区分（15株分离株有7种不同模式）。这些结果表明，MIRU-VNTR分型与间隔寡核苷酸分型和缺失分析一起，可有效地用于分子流行病学研究以确定正在传播的簇；据我们所知，这是关于孟加拉国流行菌株类型的首次报告。
