Hawkings Guy S, Galvez Fernando, Goss Greg G
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6E 4W1.
J Exp Biol. 2004 Feb;207(Pt 6):905-12. doi: 10.1242/jeb.00844.
Mitochondria-rich cells (MR cells) of the gills of rainbow trout undergo changes in relative distribution and biochemical function during acclimation to partial-strength (10 per thousand ) and full-strength (30 per thousand ) seawater. In isolated total gill cells, Na(+)/K(+)-ATPase activity increased fivefold and H(+)-ATPase activity decreased fourfold when trout were acclimated to either 10 per thousand or 30 per thousand seawater. When total MR gill cells were separated based on differential binding to peanut lectin agglutinin (PNA), the PNA subtypes underwent a change in relative distribution in seawater-acclimated fish. In freshwater, the ratio of PNA(-):PNA(+) was 65:35 while in seawater the distribution changed to 20:80 PNA(-):PNA(+). Additionally, differential changes in Na(+)/K(+)-ATPase and H(+)-ATPase activity in each of the independent cell types occurred during seawater acclimation; Na(+)/K(+)-ATPase activity in the PNA(-) cells increased by 197% while in PNA(+) cells Na(+)/K(+)-ATPase decreased by 57%. However, H(+)-ATPase activity was decreased in both PNA(-) (84%) and PNA(+) (72%) subtypes during acclimation to seawater.
虹鳟鱼鳃中富含线粒体的细胞（MR细胞）在适应半强度（10‰）和全强度（30‰）海水的过程中，其相对分布和生化功能会发生变化。当虹鳟鱼适应10‰或30‰海水时，在分离出的全鳃细胞中，Na⁺/K⁺-ATP酶活性增加了五倍，而H⁺-ATP酶活性降低了四倍。当根据与花生凝集素（PNA）的差异结合对全MR鳃细胞进行分离时，在适应海水的鱼中，PNA亚型的相对分布发生了变化。在淡水中，PNA⁻:PNA⁺的比例为65:35，而在海水中，分布变为20:80 PNA⁻:PNA⁺。此外，在海水适应过程中，每种独立细胞类型的Na⁺/K⁺-ATP酶和H⁺-ATP酶活性都发生了差异变化；PNA⁻细胞中的Na⁺/K⁺-ATP酶活性增加了197%，而PNA⁺细胞中的Na⁺/K⁺-ATP酶活性降低了57%。然而，在适应海水的过程中，PNA⁻（84%）和PNA⁺（72%）亚型的H⁺-ATP酶活性均降低。
