Dooley S, Welter C, Blin N
Institute of Human Genetics, University of Saarland, Homburg, FRG.
Biotechniques. 1992 Oct;13(4):540-3.
Replacing radioactively labeled probes by nonradioactive ones and detection by chemiluminescence instead of colorimetry allows a nonhazardous handling and offers the possibility of easily reprobing filters in Southwestern analysis. Using the described procedure, we were able to determine the molecular weight of DNA-binding proteins and achieve a high signal-to-noise ratio.
