Nonradioactive southwestern analysis using chemiluminescent detection.
作者信息
Dooley S, Welter C, Blin N
机构信息
Institute of Human Genetics, University of Saarland, Homburg, FRG.
出版信息
Biotechniques. 1992 Oct;13(4):540-3.
PMID:1476722
Abstract
Replacing radioactively labeled probes by nonradioactive ones and detection by chemiluminescence instead of colorimetry allows a nonhazardous handling and offers the possibility of easily reprobing filters in Southwestern analysis. Using the described procedure, we were able to determine the molecular weight of DNA-binding proteins and achieve a high signal-to-noise ratio.
摘要
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