Sutherland J C, Emrick A, France L L, Monteleone D C, Trunk J
Biology Department, Brookhaven National Laboratory, Upton, NY 11973.
Biotechniques. 1992 Oct;13(4):588-90.
The ultraviolet circular dichroism of a protein can be used to estimate the net fraction of its amino acids in different classes of secondary structure. Recent advances in the accuracy of such calculations have resulted from improved computational techniques, as well as extension of the spectral region analyzed to wavelengths less than 180 nm, a wavelength range beyond the limit of most laboratory-based circular dichroism spectrometers. We describe a spectrometer that uses UV radiation from the National Synchrotron Light Source at the Brookhaven National Laboratory to record circular dichroism spectra of proteins (and other biologically important molecules) in aqueous solution over the optimum wavelength range required for calculation of secondary structures. This instrument is available for use by scientists from academic, commercial and research institutions.
蛋白质的紫外圆二色性可用于估算其不同二级结构类别中氨基酸的净比例。此类计算精度的最新进展源于计算技术的改进，以及将分析的光谱区域扩展至小于180 nm的波长，该波长范围超出了大多数基于实验室的圆二色光谱仪的极限。我们描述了一种光谱仪，它利用布鲁克海文国家实验室国家同步辐射光源的紫外辐射，在计算二级结构所需的最佳波长范围内记录蛋白质（以及其他具有生物学重要性的分子）在水溶液中的圆二色光谱。该仪器可供学术、商业和研究机构的科学家使用。
