Copley C G, Boot C
Biotechnology Department, ICI Pharmaceuticals, Macclesfield, Cheshire, UK.
Biotechniques. 1992 Dec;13(6):888-92.
An assay is described in which an oligonucleotide probe is specifically digested by lambda exonuclease only when it is annealed to its complementary sequence. In this assay, a cycling effect occurs whereby a small amount of target sequence acts as a specific co-factor in the enzymatic degradation of a larger number of molecules of an oligonucleotide probe. This amplification principle is demonstrated and the effect of the oligonucleotide probe sequence investigated. The necessary steps needed to convert this effect into a useful diagnostic tool are discussed.
本文描述了一种检测方法，其中只有当寡核苷酸探针与其互补序列退火时，它才会被λ外切核酸酶特异性消化。在该检测方法中，会出现一种循环效应，即少量的靶序列在寡核苷酸探针的大量分子的酶促降解过程中作为特异性辅因子起作用。证明了这种扩增原理，并研究了寡核苷酸探针序列的影响。讨论了将这种效应转化为有用诊断工具所需的必要步骤。
