在快速凝胶中对碱性磷酸酶的化学发光检测
Chemiluminescent detection of alkaline phosphatase in PhastGel.
作者信息
Theodosiou N G, Chatot C L, Ziomek C A
机构信息
Genzyme Corporation, Framingham, MA 01701.
出版信息
Biotechniques. 1992 Dec;13(6):898-901.
PMID:1476742
Abstract
A chemiluminescent assay has been applied to the detection of alkaline phosphatase on PhastGel containing lysates of preimplantation mouse embryos. The very sensitive detection capabilities reported for the chemiluminescent system led to the investigation of its applicability to the characterization of the alkaline phosphatases in one embryo or less and to compare the sensitivity of two different commercial alkaline phosphatase chemiluminescent assays to a colorimetric assay.
摘要
一种化学发光测定法已应用于在含有植入前小鼠胚胎裂解物的PhastGel上检测碱性磷酸酶。报道的化学发光系统非常灵敏的检测能力促使人们研究其在一个或更少胚胎中碱性磷酸酶特性表征方面的适用性，并比较两种不同商业碱性磷酸酶化学发光测定法与比色测定法的灵敏度。
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