[采用离子对反相高效液相色谱法检测微卫星不稳定性]
[The detection of microsatellite instability by ion-pair reversed-phase high performance liquid chromatography].
作者信息
Liu Xiao-rong, Wang Ya-ping, Zhang Jun-ni, Li Jin-tian, Zhang Xiao-mei, Zhu Ming, Shan Xiang-nian
机构信息
Department of Molecular Biology, Jiangsu Institute of Cancer Research, Nanjing, Jiangsu, PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Feb;21(1):56-60.
OBJECTIVE
To set up a sensitive and stable technique which has high throughout to detect the instability of microsatellite DNA.
METHODS
Genomic DNA extracted from the cancer tissues and their normal tissues were subjected to microsatellite instability(MSI) analysis on five of DNA markers in 115 sporadic colorectal cancers by means of PCR and ion-pair reversed-phase high performance liquid chromatography. Genomic DNA extracted from lymphocytes in blood of 20 normal persons were analysed and used as the standard control.
RESULTS
Seventeen (14.8%) MSI-H and 23(20.0%) MSI-L were found in 115 sporadic colorectal cancers. The rates of MSI in the young patients and old patients were much higher than that in the middle-age patients (P<0.05). And the rate of MSI in low differentiation group was also much higher than that in high or middle differentiation groups (P<0.05).
CONCLUSION
The method the authors developed is a sensitive and accurate technique to detect MSI and has a high throughput.
目的
建立一种灵敏、稳定且高通量的检测微卫星DNA不稳定的技术。
方法
采用聚合酶链反应（PCR）和离子对反相高效液相色谱法，对115例散发性结直肠癌的癌组织及其正常组织提取的基因组DNA进行5个DNA标记的微卫星不稳定性（MSI）分析。对20例正常人血液中淋巴细胞提取的基因组DNA进行分析，并作为标准对照。
结果
115例散发性结直肠癌中，发现17例（14.8%）为高度微卫星不稳定（MSI-H），23例（20.0%）为低度微卫星不稳定（MSI-L）。年轻患者和老年患者的MSI发生率明显高于中年患者（P<0.05）。低分化组的MSI发生率也明显高于高分化或中分化组（P<0.05）。
结论
作者建立的方法是一种检测MSI的灵敏、准确且高通量的技术。
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