Liu Zhong-min, Guan Zhao-jie, Shen Wen-lv
Department of Surgery, Medical College, Shantou University, Shantou, Guangdong, P. R. China 515041.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 Jan;18(1):63-5.
To simplify surgical technique and increase postoperative survival rate, sleeve anastomosis technique combined cuff technique was used in developing the model of cervical heart transplantation in rats.
In this model, the hearts from 25 male SD rats were transplanted into the neck of Wistar rats by anastomosing the donor innominate artery to the recipient right common carotid artery by use of sleeve technique, and the donor pulmonary artery to the recipient right external jugular vein by use of cuff technique. After operation, the rats were treated with cyclosporine A (1.5 mg/kg, q.d.), transplanted hearts were followed by daily inspection or palpation and the allograft survival time was more than 3 days as the standard of successful operation.
The mean operative time was (48.7 +/- 3.4) min, with a successful rate of 88% (22/25). Complications were anastomotic hemorrhage (1 case) and thrombosis(2 cases). During the follow-up period, 6 rats died of pulmonary infection, abscess in the neck, liver or bladder tumor. The remaining 16 transplanted hearts survived more than 3 months.
The modified operation have advantages of less operative procedure, shorter operation and ischemia time and easier monitoring of graft function.
为简化手术技术并提高术后生存率，在大鼠颈部心脏移植模型构建中采用袖套吻合技术联合套扎技术。
在此模型中，将25只雄性SD大鼠的心脏移植到Wistar大鼠颈部，采用袖套技术将供体无名动脉与受体右颈总动脉吻合，采用套扎技术将供体肺动脉与受体右颈外静脉吻合。术后，大鼠接受环孢素A（1.5mg/kg，每日一次）治疗，对移植心脏每日进行检查或触诊，以移植心脏存活时间超过3天作为手术成功标准。
平均手术时间为（48.7±3.4）分钟，成功率为88%（22/25）。并发症为吻合口出血（1例）和血栓形成（2例）。随访期间，6只大鼠死于肺部感染、颈部脓肿、肝脏或膀胱肿瘤。其余16个移植心脏存活超过3个月。
改良手术具有手术步骤少、手术及缺血时间短、移植心脏功能监测更容易等优点。
