Zhu L, Gong D, Zou Y, Li Y, Wu Y, Yuan B, Guan L, Wu Q
Department of Physiology, Dalian Medical University, Dalian, China.
Transplant Proc. 2008 Jun;40(5):1645-9. doi: 10.1016/j.transproceed.2008.03.146.
Our goal was to establish a simple and stable cervical heterotopic small intestinal transplantation (SIT) rat model that minimizes mortality.
The graft was removed en bloc consisting of small intestine, portal vein (PV), and donor superior mesenteric artery. The graft was perfused in situ and the gut lumen irrigated during the operation. The donor artery was end-to-end anastomosed to the recipient left common carotid artery using a sleeve technique, and the donor vein to the recipient right external jugular vein using a cuff technique. The proximal and distal ends of the graft were formed as cervical cutaneous stomas.
Among 106 intestinal transplantations, 40 were a formal experimental group, displaying a survival rate of 95%. Surgery lasted 70 +/- 10 minutes. The average time for the arterial anastomosis and the venous anastomosis was 5 +/- 2 minutes and 2 +/- 1 minutes, respectively. Two rats died due to late anastomotic hemorrhage or intestinal fistula; 38 survived more than 6 months.
The cervical heterotopic technique has the advantages of being easier and faster to perform with shorter cold ischemia time and higher graft survival rate as well as fewer complications.
我们的目标是建立一种简单且稳定的颈异位小肠移植（SIT）大鼠模型，使死亡率降至最低。
将包含小肠、门静脉（PV）和供体肠系膜上动脉的移植物整体取出。在手术过程中对移植物进行原位灌注并冲洗肠腔。采用套管技术将供体动脉与受体左颈总动脉进行端端吻合，采用袖套技术将供体静脉与受体右颈外静脉进行吻合。移植物的近端和远端形成颈部皮肤造口。
在106例小肠移植中，40例为正式实验组，存活率为95%。手术持续70±10分钟。动脉吻合和静脉吻合的平均时间分别为5±2分钟和2±1分钟。2只大鼠因晚期吻合口出血或肠瘘死亡；38只存活超过6个月。
颈异位技术具有操作更容易、更快，冷缺血时间短，移植物存活率高以及并发症少等优点。
