Nakamura H, Ozawa H
Department of Oral Anatomy, Niigata University School of Dentistry, Japan.
Bone. 1992;13(6):411-6. doi: 10.1016/8756-3282(92)90083-9.
Lectin cytochemistry was performed to clarify the process of glycosylation and the localization of glycocalyx in osteoclasts. Microslicer sections of decalcified rat tibiae were incubated in the presence of HRP-conjugated lectins (Con A, PNA, MPA, WGA, UEA-1). Lectin reactions in cell organelles revealed that glucose (Glc) and mannose (Man) are transferred to carbohydrate chains in nuclear envelopes, rough endoplasmic reticuli, and the cis and medial sides of the Golgi apparatus. N-acetylgalactosamine (GalNAc), N-acetylglucosamine (GlcNAc), and/or N-acetylneuraminic acic (NANA) residues are transferred, in turn, in the Golgi apparatus. Lectin reactions detected in lysosomal structures suggest that some sugar residues are incorporated into carbohydrate chains of hydrolytic enzymes, such as acid phosphatase and arylsulfatase. Others would be transported to plasma membranes as glycocalyx. PNA and MPA reactions were most evident on ruffled borders of osteoclasts. On the other hand, cement-line-like structures on bone surfaces displayed Con A, MPA, and WGA positive reactions. The following factors suggest that osteoclasts actively metabolize sugar: characteristic localization of glycocalyx in osteoclasts reflect the polarity of osteoclasts, and carbohydrate complexes in cement-line-like structures seem to play an important role in the coupling phenomenon in bone tissue.
进行凝集素细胞化学实验以阐明破骨细胞中糖基化过程及糖萼的定位。将脱钙大鼠胫骨的微切片在与辣根过氧化物酶（HRP）偶联的凝集素（刀豆球蛋白A、花生凝集素、麦芽凝集素、小麦胚凝集素、荆豆凝集素-1）存在的情况下孵育。细胞器中的凝集素反应显示，葡萄糖（Glc）和甘露糖（Man）被转移至核膜、糙面内质网以及高尔基体顺面和中间面的碳水化合物链上。N-乙酰半乳糖胺（GalNAc）、N-乙酰葡糖胺（GlcNAc）和/或N-乙酰神经氨酸（NANA）残基则依次在高尔基体中被转移。在溶酶体结构中检测到的凝集素反应表明，一些糖残基被并入水解酶（如酸性磷酸酶和芳基硫酸酯酶）的碳水化合物链中。其他糖残基则作为糖萼被转运至质膜。花生凝集素和麦芽凝集素反应在破骨细胞的皱褶缘最为明显。另一方面，骨表面的类黏合线结构显示刀豆球蛋白A、麦芽凝集素和小麦胚凝集素呈阳性反应。以下因素表明破骨细胞能积极代谢糖类：破骨细胞中糖萼的特征性定位反映了破骨细胞的极性，且类黏合线结构中的碳水化合物复合物似乎在骨组织的耦联现象中发挥重要作用。
