Fonseca Fonseca Lisandra, Díaz Ana María
Department of Medical Technology, College of Health Allied Professions, Medical Sciences Campus, University of Puerto Rico, PO Box 365067, San Juan, Puerto Rico 00936-5067.
P R Health Sci J. 2003 Dec;22(4):353-7.
To determine the IgE reactivity against recombinant protein Blo t 1 from the dust mite Blomia tropicalis (Bt) using serum from patients with positive skin test to this mite and to investigate the cross-reactivity between Bt and Dermatophagoides pteronyssinus.
Dust mites have an important role as inducers of allergic asthma and rhinitis. Particularly, Bt is an important mite specie in the tropic and subtropical regions of the world. Therefore, recombinant allergens of this organism could be an important feature for development of effective diagnostic and therapeutic measures.
Purified recombinant Blot t 1 was produced in Escherichia coli and tested against sera from 54 allergic individuals by the dot blot technique.
IgE response to Blot 1 was 72% for sera from patients with positive skin test to Bt. Cross-reactivity with Dermatophagoides pteronyssinus was not detected. Statistical analyses of the dot blot test results show 71.74% of sensitivity and 100% of specificity.
By using a panel of allergic sera and an in vitro assay, the allergen rBlo t 1 exhibits no IgE cross-reactivity with Dermathophagoides pteronyssinus allergens. This finding suggests that specific clinical reagents are necessary for precise diagnosis and treatment of sensitization to Bt.
使用对热带无爪螨（Bt）皮肤试验呈阳性的患者血清，测定其对热带无爪螨重组蛋白Blo t 1的IgE反应性，并研究Bt与屋尘螨之间的交叉反应性。
尘螨是变应性哮喘和鼻炎的重要诱发因素。特别是，Bt是世界热带和亚热带地区的一种重要螨类。因此，该生物体的重组变应原可能是开发有效诊断和治疗措施的重要特征。
在大肠杆菌中产生纯化的重组Blot t 1，并通过斑点印迹技术对54名过敏个体的血清进行检测。
对Bt皮肤试验呈阳性的患者血清对Blot 1的IgE反应率为72%。未检测到与屋尘螨的交叉反应性。斑点印迹试验结果的统计分析显示敏感性为71.74%，特异性为100%。
通过使用一组过敏血清和体外试验，变应原rBlo t 1与屋尘螨变应原无IgE交叉反应性。这一发现表明，对于精确诊断和治疗对Bt的致敏，需要特定的临床试剂。
