Finkel'shteĭn Z I, Baskunov B P, Golovlev E L, Vervoort J, Rietjens I M, Baboshin M A, Golovleva L A
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
Mikrobiologiia. 2003 Nov-Dec;72(6):746-51.
Of the four investigated Rhodococcus strains (R. rhodochrous 172, R. opacus 4a and 557, and R. rhodnii 135), the first three strains were found to be able to completely transform fluorene when it was present in the medium as the sole source of carbon at a concentration of 12-25 mg/l. At a fluorene concentration of 50-100 mg/l in the medium, the rhodococci transformed 50% of the substrate in 14 days. The addition of casamino acids and sucrose (1-5 g/l) stimulated fluorene transformation, so that R. rhodochrous 172 could completely transform it in 2-5 days. Nine intermediates of fluorene transformation were isolated, purified, and structurally characterized. It was found that R. rhodnii 135 and R. opacus strains 4a and 557 hydroxylated fluorene with the formation of 2-hydroxyfluorene and 2,7-dihydroxyfluorene. R. rhodochrous 172 transformed fluorene via two independent pathways to a greater degree than did the other rhodococci studied.
在所研究的四株红球菌菌株（红平红球菌172、奥氏红球菌4a和557以及红迪红球菌135）中，发现前三株菌株能够在芴以12 - 25毫克/升的浓度作为培养基中唯一碳源存在时将其完全转化。当培养基中芴的浓度为50 - 100毫克/升时，红球菌在14天内将50%的底物转化。添加酪蛋白氨基酸和蔗糖（1 - 5克/升）刺激了芴的转化，使得红平红球菌172能够在2 - 5天内将其完全转化。分离、纯化并对芴转化的九种中间体进行了结构表征。发现红迪红球菌135以及奥氏红球菌4a和557菌株使芴羟基化，生成2 - 羟基芴和2,7 - 二羟基芴。红平红球菌172比所研究的其他红球菌通过两条独立途径转化芴的程度更高。
