Kataranovski M, Kucuk J, Lilić D, Drasković-Pavlović B, Colić M, Pejnović N, Dujić A
Department of Clinical and Experimental Immunology, Institute for Medical Research, Military Medical Academy, Belgrade, Yugoslavia.
Reg Immunol. 1992 Jul-Aug;4(4):197-203.
While trauma-induced suppression of T-cell responses is well documented, only few studies address lymphocyte activation. The aim of this study was to investigate the functional activity of lymph node cells in rats subjected to scald injury, proliferative activity, cytokine production, and responsiveness to exogenously added cytokines was evaluated in cells from lymph nodes draining burned tissue and from distant, nondraining lymph node cells. Results presented clearly show that lymph node cells in scalded rats are activated in vivo although the extent of proliferation and pattern of cytokine production differ: a) proliferative activity was elevated both in draining and distant lymph nodes, but was more pronounced in cells from lymph nodes draining the injured region; b) increased production of IL-2, and particularly IL-1 and IL-6 was found and coincides well with peak of proliferative activity of draining lymph node cells; IL-2 production by distant lymph node cells remained unchanged, IL-1 and IL-6 production was significantly increased coinciding with increased proliferation; c) increased responsiveness to exogenously supplied cytokines was found in draining lymph node cells, while it remained unchanged in nondraining lymph node cells. Early activation of lymphocytes demonstrated in our experiments could be one of the previously unrecognized consequences of trauma-induced immunosuppression.
虽然创伤诱导的T细胞反应抑制已有充分记录，但仅有少数研究涉及淋巴细胞活化。本研究的目的是调查烫伤大鼠淋巴结细胞的功能活性，评估烧伤组织引流淋巴结细胞和远处非引流淋巴结细胞的增殖活性、细胞因子产生情况以及对外源性添加细胞因子的反应性。呈现的结果清楚地表明，烫伤大鼠的淋巴结细胞在体内被激活，尽管增殖程度和细胞因子产生模式有所不同：a）引流淋巴结和远处淋巴结的增殖活性均升高，但在损伤区域引流淋巴结的细胞中更为明显；b）发现IL-2、特别是IL-1和IL-6的产生增加，且与引流淋巴结细胞增殖活性的峰值高度吻合；远处淋巴结细胞的IL-2产生保持不变，IL-1和IL-6产生随着增殖增加而显著增加；c）引流淋巴结细胞对外源性供应细胞因子的反应性增加，而非引流淋巴结细胞则保持不变。我们实验中证明的淋巴细胞早期活化可能是创伤诱导免疫抑制先前未被认识的后果之一。
