比较两种测量氟喹诺酮类药物光遗传毒性诱导的DNA损伤的方法。

Compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones.

作者信息

Zhang Ting, Li Jun-ling, Xin Jian, Ma Xiao-chao, Tu Zeng-hong

机构信息

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.

出版信息

Acta Pharmacol Sin. 2004 Feb;25(2):171-5.

PMID:14769204

Abstract

AIM

To compare two methods of measuring DNA damage induced by photogenotoxicity of fluoroquinolones (FQ).

METHODS

Lomefloxacin (LFLX), sparfloxacin (SPFX), ciprofloxacin (CPFX), and levofloxacin (LELX) were tested by comet assay and photodynamic DNA strand breaking activity under the different conditions of UVA irradiation.

RESULTS

In comet assay, photogenotoxicity was evident at SPFX 1 mg/L, LFLX 5 mg/L, and CPFX 5 mg/L, and LELX 10 mg/L. In photodynamic DNA strand-breaking activity, SPFX and LFLX induced the conversion of the supercoiled form into the nicked relaxed form at 10-50 micromol/L, while CPFX at 25 micromol/L and LELX at 50 micromol/L.

CONCLUSION

There were good correlations between the two methods to detect DNA damage induced by phototoxicity of fluoroquinolones. Photodynamic DNA strand breaking activity was a good method to detect DNA damage induced by photogenotoxicity of fluoroquinolones as well as comet assay.

摘要

目的

比较两种检测氟喹诺酮类（FQ）光遗传毒性诱导的DNA损伤的方法。

方法

通过彗星试验以及在不同UVA照射条件下的光动力DNA链断裂活性，对洛美沙星（LFLX）、司帕沙星（SPFX）、环丙沙星（CPFX）和左氧氟沙星（LELX）进行检测。

结果

在彗星试验中，在司帕沙星1mg/L、洛美沙星5mg/L、环丙沙星5mg/L和左氧氟沙星10mg/L时，光遗传毒性明显。在光动力DNA链断裂活性试验中，司帕沙星和洛美沙星在10 - 50微摩尔/升时诱导超螺旋形式转变为切口松弛形式，而环丙沙星在25微摩尔/升时以及左氧氟沙星在50微摩尔/升时出现这种转变。