Schuller Dorit, Valero Eva, Dequin Sylvie, Casal Margarida
Departamento de Biologia, Campus de Gualtar, Universidade do Minho, 4710-057 Braga, Portugal.
FEMS Microbiol Lett. 2004 Feb 9;231(1):19-26. doi: 10.1016/S0378-1097(03)00928-5.
A survey of the genetic polymorphisms produced by distinct methods was performed in 23 commercial winery yeast strains. Microsatellite typing, using six different loci, an optimized interdelta sequence analysis and restriction fragment length polymorphism of mitochondrial DNA generated by the enzyme HinfI had the same discriminatory power: among the 23 commercial yeast strains, 21 distinct patterns were obtained. Karyotype analysis gave 22 patterns, thereby allowing the discrimination of one of the three strains that were not distinguished by the other methods. Due to the equivalence of the results obtained in this survey, any of the methods can be applied at the industrial scale.
对23种商业酿酒酵母菌株中不同方法产生的遗传多态性进行了调查。使用六个不同位点的微卫星分型、优化的δ序列间分析以及由HinfI酶产生的线粒体DNA限制性片段长度多态性具有相同的鉴别能力：在23种商业酵母菌株中，获得了21种不同的模式。核型分析给出了22种模式，从而能够区分出其他方法无法区分的三种菌株中的一种。由于本次调查获得的结果具有等效性，因此任何一种方法都可应用于工业规模。
