Cinar Ozer
Kahramanmaraş Sütçü Imam University, Department of Environmental Engineering, Karacasu, Kahramanmaraş 46160, Turkey.
FEMS Microbiol Lett. 2004 Feb 9;231(1):59-65. doi: 10.1016/S0378-1097(03)00920-0.
The response of a mixed microbial culture to different feed compositions, that is, containing benzoate and pyruvate as sole carbon sources at different levels, was studied in a chemostat with a 48-h hydraulic residence time under cyclic aerobic and anoxic (denitrifying) conditions. The cyclic bacterial culture was well adapted to different feed compositions as evidenced by the lack of accumulation of benzoate or pyruvate in the chemostat. Both the benzoate-degrading capabilities and the in vitro catechol 2,3-dioxygenase (C23DO) activities of the cyclic bacterial cultures were in direct proportion to the flux through the chemostat of the substrate degraded by the pathway containing C23DO, with some exceptions. The quantity of C23DO showed a transient decrease during the initial portion of the aerobic period before returning to the level present during the anoxic period. That decrease was most likely caused by the production of H(2)O(2) by the cells upon being returned to aerobic conditions.
在水力停留时间为48小时的恒化器中，在循环好氧和缺氧（反硝化）条件下，研究了混合微生物培养物对不同进料组成（即含有不同水平的苯甲酸盐和丙酮酸盐作为唯一碳源）的响应。循环细菌培养物能很好地适应不同的进料组成，这可通过恒化器中苯甲酸盐或丙酮酸盐没有积累得到证明。循环细菌培养物的苯甲酸盐降解能力和体外儿茶酚2,3-双加氧酶（C23DO）活性都与通过含有C23DO途径降解的底物在恒化器中的通量成正比，但有一些例外。C23DO的量在好氧期开始阶段出现短暂下降，然后才恢复到缺氧期时的水平。这种下降很可能是由于细胞在恢复到好氧条件时产生了H(2)O(2)。
