Celotto Alicia M, Graveley Brenton R
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, USA.
Methods Mol Biol. 2004;257:245-54. doi: 10.1385/1-59259-750-5:245.
RNA interference (RNAi) is a useful tool for degrading targeted messenger RNAs (mRNAs) and thus "knocking down" the abundance of the encoded protein. We have been using RNAi in cultured Drosophila cells to evaluate the effect of "knocking down" numerous mRNA processing factors on the alternative splicing of specific pre-mRNAs. This relatively simple technique has allowed us to identify a number of splicing factors that impact the alternative splicing of particular alternatively spliced exons. This approach can be extended to examine the splicing of nearly any gene.
RNA干扰（RNAi）是一种用于降解靶向信使核糖核酸（mRNA）从而“敲低”编码蛋白质丰度的有用工具。我们一直在培养的果蝇细胞中使用RNAi来评估“敲低”众多mRNA加工因子对特定前体mRNA可变剪接的影响。这种相对简单的技术使我们能够鉴定出许多影响特定可变剪接外显子可变剪接的剪接因子。这种方法可以扩展到检查几乎任何基因的剪接。
