果蝇S2细胞中mRNA加工因子的RNA干扰
RNA interference of mRNA processing factors in Drosophila S2 cells.
作者信息
Celotto Alicia M, Graveley Brenton R
机构信息
Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, USA.
出版信息
Methods Mol Biol. 2004;257:245-54. doi: 10.1385/1-59259-750-5:245.
Abstract
RNA interference (RNAi) is a useful tool for degrading targeted messenger RNAs (mRNAs) and thus "knocking down" the abundance of the encoded protein. We have been using RNAi in cultured Drosophila cells to evaluate the effect of "knocking down" numerous mRNA processing factors on the alternative splicing of specific pre-mRNAs. This relatively simple technique has allowed us to identify a number of splicing factors that impact the alternative splicing of particular alternatively spliced exons. This approach can be extended to examine the splicing of nearly any gene.
摘要
RNA干扰（RNAi）是一种用于降解靶向信使核糖核酸（mRNA）从而“敲低”编码蛋白质丰度的有用工具。我们一直在培养的果蝇细胞中使用RNAi来评估“敲低”众多mRNA加工因子对特定前体mRNA可变剪接的影响。这种相对简单的技术使我们能够鉴定出许多影响特定可变剪接外显子可变剪接的剪接因子。这种方法可以扩展到检查几乎任何基因的剪接。
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本文引用的文献
1
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2
Alternative splicing and genome complexity.可变剪接与基因组复杂性。
Nat Genet. 2002 Jan;30(1):29-30. doi: 10.1038/ng803. Epub 2001 Dec 17.
3
Modulation of P-element pre-mRNA splicing by a direct interaction between PSI and U1 snRNP 70K protein.通过PSI与U1 snRNP 70K蛋白之间的直接相互作用对P因子前体mRNA剪接进行调控。
Mol Cell. 2001 Aug;8(2):363-73. doi: 10.1016/s1097-2765(01)00311-2.
4
RNA interference: listening to the sound of silence.RNA干扰：聆听沉默之声。
Nat Struct Biol. 2001 Sep;8(9):746-50. doi: 10.1038/nsb0901-746.
5
RNA interference is mediated by 21- and 22-nucleotide RNAs.RNA干扰由21和22个核苷酸的RNA介导。
Genes Dev. 2001 Jan 15;15(2):188-200. doi: 10.1101/gad.862301.
6
Heritable gene silencing in Drosophila using double-stranded RNA.利用双链RNA在果蝇中实现可遗传的基因沉默。
Nat Biotechnol. 2000 Aug;18(8):896-8. doi: 10.1038/78531.
7
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8
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9
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