黑腹果蝇的基因组序列。

The genome sequence of Drosophila melanogaster.

作者信息

Adams M D, Celniker S E, Holt R A, Evans C A, Gocayne J D, Amanatides P G, Scherer S E, Li P W, Hoskins R A, Galle R F, George R A, Lewis S E, Richards S, Ashburner M, Henderson S N, Sutton G G, Wortman J R, Yandell M D, Zhang Q, Chen L X, Brandon R C, Rogers Y H, Blazej R G, Champe M, Pfeiffer B D, Wan K H, Doyle C, Baxter E G, Helt G, Nelson C R, Gabor G L, Abril J F, Agbayani A, An H J, Andrews-Pfannkoch C, Baldwin D, Ballew R M, Basu A, Baxendale J, Bayraktaroglu L, Beasley E M, Beeson K Y, Benos P V, Berman B P, Bhandari D, Bolshakov S, Borkova D, Botchan M R, Bouck J, Brokstein P, Brottier P, Burtis K C, Busam D A, Butler H, Cadieu E, Center A, Chandra I, Cherry J M, Cawley S, Dahlke C, Davenport L B, Davies P, de Pablos B, Delcher A, Deng Z, Mays A D, Dew I, Dietz S M, Dodson K, Doup L E, Downes M, Dugan-Rocha S, Dunkov B C, Dunn P, Durbin K J, Evangelista C C, Ferraz C, Ferriera S, Fleischmann W, Fosler C, Gabrielian A E, Garg N S, Gelbart W M, Glasser K, Glodek A, Gong F, Gorrell J H, Gu Z, Guan P, Harris M, Harris N L, Harvey D, Heiman T J, Hernandez J R, Houck J, Hostin D, Houston K A, Howland T J, Wei M H, Ibegwam C, Jalali M, Kalush F, Karpen G H, Ke Z, Kennison J A, Ketchum K A, Kimmel B E, Kodira C D, Kraft C, Kravitz S, Kulp D, Lai Z, Lasko P, Lei Y, Levitsky A A, Li J, Li Z, Liang Y, Lin X, Liu X, Mattei B, McIntosh T C, McLeod M P, McPherson D, Merkulov G, Milshina N V, Mobarry C, Morris J, Moshrefi A, Mount S M, Moy M, Murphy B, Murphy L, Muzny D M, Nelson D L, Nelson D R, Nelson K A, Nixon K, Nusskern D R, Pacleb J M, Palazzolo M, Pittman G S, Pan S, Pollard J, Puri V, Reese M G, Reinert K, Remington K, Saunders R D, Scheeler F, Shen H, Shue B C, Sidén-Kiamos I, Simpson M, Skupski M P, Smith T, Spier E, Spradling A C, Stapleton M, Strong R, Sun E, Svirskas R, Tector C, Turner R, Venter E, Wang A H, Wang X, Wang Z Y, Wassarman D A, Weinstock G M, Weissenbach J, Williams S M, Worley K C, Wu D, Yang S, Yao Q A, Ye J, Yeh R F, Zaveri J S, Zhan M, Zhang G, Zhao Q, Zheng L, Zheng X H, Zhong F N, Zhong W, Zhou X, Zhu S, Zhu X, Smith H O, Gibbs R A, Myers E W, Rubin G M, Venter J C

机构信息

Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.

出版信息

Science. 2000 Mar 24;287(5461):2185-95. doi: 10.1126/science.287.5461.2185.

DOI:10.1126/science.287.5461.2185

PMID:10731132

Abstract

The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans. We have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map. Efforts are under way to close the remaining gaps; however, the sequence is of sufficient accuracy and contiguity to be declared substantially complete and to support an initial analysis of genome structure and preliminary gene annotation and interpretation. The genome encodes approximately 13,600 genes, somewhat fewer than the smaller Caenorhabditis elegans genome, but with comparable functional diversity.

摘要

果蝇是生物学中研究最为深入的生物之一，作为一个模型系统，用于研究包括人类在内的高等真核生物许多常见的发育和细胞过程。我们采用全基因组鸟枪法测序策略，并辅以大量基于克隆的序列和高质量的细菌人工染色体物理图谱，确定了果蝇基因组约120兆碱基常染色质部分几乎所有的核苷酸序列。目前正在努力填补剩余的缺口；然而，该序列具有足够的准确性和连续性，可宣布基本完成，并支持对基因组结构的初步分析以及初步的基因注释和解读。该基因组编码约13600个基因，比小一些的秀丽隐杆线虫基因组略少，但功能多样性相当。