Keljo D J, Kleinzeller A, Murer H, Kinne R
Biochim Biophys Acta. 1978 Apr 20;508(3):500-12. doi: 10.1016/0005-2736(78)90095-0.
The possible presence of hexokinase in basal lateral membranes from rat kidney proximal tubules was investigated. Basal lateral membranes were obtained from homogenates of rat kidney cortex by differential centrifugation and free flow electrophoresis. They were further purified by density gradient centrifugation. Hexokinase activity was measured as the phosphorylation of D-[U14C]glucose. Throughout the purification of the membranes, the specific activity of hexokinase decreased while that of (Na+ + K+)-ATPase increased. Hexokinase activity in all fractions could be quantitatively accounted for in terms of cytosolic and mitochondrial enzyme contributions. It is concluded that there is no hexokinase activity in basal lateral membranes from rat kidney.
研究了大鼠肾近端小管基底外侧膜中己糖激酶可能的存在情况。通过差速离心和自由流动电泳从大鼠肾皮质匀浆中获得基底外侧膜。它们通过密度梯度离心进一步纯化。己糖激酶活性通过D-[U14C]葡萄糖的磷酸化来测定。在整个膜的纯化过程中，己糖激酶的比活性降低，而(Na+ + K+)-ATP酶的比活性增加。所有组分中的己糖激酶活性可以根据胞质和线粒体酶的贡献进行定量解释。得出的结论是，大鼠肾基底外侧膜中不存在己糖激酶活性。
