Mani S K, Julian J, Lampelo S, Glasser S R
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Biol Reprod. 1992 Nov;47(5):785-99. doi: 10.1095/biolreprod47.5.785.
The effects of in vivo hormonal sensitization on the competence of uterine stromal (US) cells to decidualize in vitro were assessed. In vitro differentiation of uterine stroma isolated from Day 4 pregnant rats, sensitized to respond to a decidual stimulus, was compared to that in nonsensitized immature, castrated or cycling rats. The initiation of in vitro decidualization--as monitored by the expression of the decidual markers desmin and laminin in rat US cells--was independent of the hormonal status of the animal from which the cells were isolated and occurred in the absence of serum in the medium. Differentiation was accelerated in high-density cultures where contact inhibition suppressed proliferation and decreased the extent of cell growth. The extent to which in vitro decidualization imitates in vivo stromal cell differentiation was assessed by comparing decidualization in the rabbit, a species with only a limited decidual cell response, and in the rat. US cells isolated from nonpregnant rabbits differentiated in vitro by expressing laminin, but not desmin. Indirect immunofluorescence of frozen uterine sections from pregnant and nonpregnant rabbits validated in vitro differentiation as a faithful reflection of the in vivo program of decidualization. Although the program of US cell differentiation may vary between the species, initiation of differentiation in vitro appeared to be independent of hormonal preparation in vivo for both the species examined.
评估了体内激素致敏对子宫基质（US）细胞体外蜕膜化能力的影响。将从第4天妊娠大鼠分离的、对蜕膜刺激有反应的致敏子宫基质的体外分化，与未致敏的未成熟、去势或动情周期大鼠的子宫基质的体外分化进行比较。体外蜕膜化的起始——通过大鼠US细胞中蜕膜标志物结蛋白和层粘连蛋白的表达来监测——与分离细胞的动物的激素状态无关，并且在培养基中无血清的情况下发生。在高密度培养中，接触抑制抑制了增殖并降低了细胞生长程度，从而加速了分化。通过比较兔（一种蜕膜细胞反应有限的物种）和大鼠的蜕膜化，评估了体外蜕膜化模拟体内基质细胞分化的程度。从未孕兔分离的US细胞通过表达层粘连蛋白而非结蛋白在体外分化。对孕兔和未孕兔子宫冰冻切片的间接免疫荧光验证了体外分化是体内蜕膜化程序的真实反映。尽管US细胞分化程序在不同物种之间可能有所不同，但在所研究的两个物种中，体外分化的起始似乎都与体内激素制备无关。
