AGNER K
J Exp Med. 1950 Oct 1;92(4):337-47. doi: 10.1084/jem.92.4.337.
The mechanism of the peroxidative oxidation of diphtheria toxin has been investigated. It has been found that this reaction requires the presence of an oxidizable, dialyzable cofactor in addition to hydrogen peroxide and peroxidase. Cofactors are present in a variety of materials and have been partially purified from urine. The flocculation titer of toxin modified by peroxidation remains unchanged even after 99 per cent or more of the toxicity has been destroyed. The change thus appears analogous to the conversion of toxin to toxoid in the presence of formalin. It is suggested that the well known conversion of crude diphtheria toxin to toxoid which occurs on storage or aging may be due to a slow peroxidative process.
对白喉毒素的过氧化氧化机制进行了研究。已发现该反应除了需要过氧化氢和过氧化物酶外,还需要一种可氧化的、可透析的辅因子。辅因子存在于多种物质中,并且已从尿液中部分纯化出来。即使在99%或更多的毒性被破坏后,经过氧化修饰的毒素的絮凝效价仍保持不变。因此,这种变化似乎类似于在福尔马林存在下毒素向类毒素的转化。有人提出,粗制白喉毒素在储存或老化过程中发生的向类毒素的众所周知的转化可能是由于缓慢的过氧化过程。