Westlin L M, Millar R P
Mammal Research Institute, University of Pretoria, South Africa.
Gen Comp Endocrinol. 1992 Nov;88(2):253-60. doi: 10.1016/0016-6480(92)90257-k.
Regulation of corpus luteum function was studied in pouched mice, housed under controlled conditions (14L:10D), (22 +/- 2 degrees C). Prolactin in daily doses of 32 IU promoted luteal and uterine development and markedly increased plasma levels of progesterone in females exhibiting estrous cycles. Negligible levels of progesterone in prolactin-treated ovariectomized females ruled out the possibility of a significant nonovarian source of progesterone. Domperidone (a dopamine antagonist) induced pseudopregnancy in cycling females, with a marked increase of peripheral progesterone levels. Pseudopregnancy was also induced by artificial vaginal stimulation of cycling females according to a multiple intromission-multiple ejaculation pattern or a shorter, more concentrated stimulation pattern. The latter treatment, however, resulted in a much weaker histological response of the corpora lutea and uterine horns, although plasma levels of progesterone were similar in the two treatment groups. Recently mated females given domperidone daily did not have progesterone levels above those of mated females treated with vehicle only (both groups pregnant). Bromocriptine (a dopamine agonist) prevented newly mated females from becoming pregnant and interrupted pregnancy when given from Day 6 onward. The progesterone level in these females was lowered. A GnRH-antagonist given to ovariectomized females significantly suppressed LH levels, but intact females given artificial vaginal stimulation and treated with an GnRH antagonist became pseudopregnant. These findings indicate that, in the pouched mouse, as in many other rodent species, pseudopregnancy and progesterone production are dependent on prolactin and not on LH.
在处于受控条件（14小时光照：10小时黑暗）、（22±2摄氏度）下饲养的有袋小鼠中研究了黄体功能的调节。每日剂量为32国际单位的催乳素促进了黄体和子宫的发育，并显著提高了处于发情周期的雌性小鼠血浆中孕酮的水平。在接受催乳素治疗的去卵巢雌性小鼠中，孕酮水平可忽略不计，排除了孕酮有重要非卵巢来源的可能性。多潘立酮（一种多巴胺拮抗剂）在处于发情周期的雌性小鼠中诱导假孕，外周孕酮水平显著升高。根据多次插入-多次射精模式或更短、更集中的刺激模式，对处于发情周期的雌性小鼠进行人工阴道刺激也可诱导假孕。然而，后一种处理导致黄体和子宫角的组织学反应弱得多，尽管两个处理组的血浆孕酮水平相似。每天给予多潘立酮的近期交配雌性小鼠的孕酮水平并不高于仅接受赋形剂处理的交配雌性小鼠（两组均怀孕）。溴隐亭（一种多巴胺激动剂）可防止新交配的雌性小鼠怀孕，并在第6天以后给予时中断妊娠。这些雌性小鼠的孕酮水平降低。给去卵巢雌性小鼠注射促性腺激素释放激素拮抗剂可显著抑制促黄体生成素水平，但对接受人工阴道刺激并接受促性腺激素释放激素拮抗剂治疗的完整雌性小鼠进行处理后，它们会进入假孕状态。这些发现表明，在有袋小鼠中，与许多其他啮齿动物物种一样，假孕和孕酮的产生依赖于催乳素而不是促黄体生成素。
