用于检测骨感染中金黄色葡萄球菌胞外蛋白抗体的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for the detection of antibodies to exocellular proteins of Staphylococcus aureus in bone infection.
作者信息
Lambert P A, Krikler S J, Patel R, Parvathan S
机构信息
Pharmaceutical Sciences Institute, Aston University, Birmingham, UK.
出版信息
FEMS Microbiol Lett. 1992 Dec 15;100(1-3):67-70. doi: 10.1111/j.1574-6968.1992.tb14020.x.
An enzyme-linked immunosorbent assay (ELISA) was developed for measurement of the antibody response to exocellular protein antigens of Staphylococcus aureus. The wells of a microtitre plate were coated with the exocellular proteins present in the tryptic soya broth growth medium from a stationary phase culture of a bone infection strain of S. aureus. The wells were then reacted sequentially with patient sera, protein A-peroxidase conjugate and chromogenic substrate. Serum from patients with S. aureus bone infection gave a significantly higher IgG titre than sera from patients with Staphylococcus epidermidis or Streptococcus sanguis bone infection or healthy uninfected individuals. The assay therefore appears to have potential in the serodiagnosis of S. aureus bone infection.
开发了一种酶联免疫吸附测定法（ELISA），用于测量对金黄色葡萄球菌细胞外蛋白抗原的抗体反应。微量滴定板的孔用来自金黄色葡萄球菌骨感染菌株的胰蛋白酶大豆肉汤生长培养基中的细胞外蛋白包被。然后将孔依次与患者血清、蛋白A-过氧化物酶缀合物和显色底物反应。金黄色葡萄球菌骨感染患者的血清IgG滴度明显高于表皮葡萄球菌或血链球菌骨感染患者或健康未感染个体的血清。因此，该测定法在金黄色葡萄球菌骨感染的血清诊断中似乎具有潜力。
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