Doskow J R, Wilkinson M F
Microbiology and Immunology Dept., Oregon Health Services University, Portland 97201.
Immunology. 1992 Nov;77(3):465-8.
CD3 proteins transduce signals delivered from the T-cell receptor (TcR) for antigen. The genes that encode the individual CD3 subunits are asynchronously regulated in tumour cell lines in vitro. In this report, we examined the expression of individual CD3 and TcR genes during normal murine thymocyte ontogeny in vivo. We show that CD3-gamma, -delta, -epsilon, and zeta transcripts are all expressed on Day 14 post-coitum (p.c.), along with IL-2R alpha and Thy-1 mRNA, and prior to the expression of functional TcR-alpha, -beta, CD4 and CD8 transcripts. Individual CD3 subunits display unique patterns of increased gene expression as ontogeny proceeds. Unique regulation of these T-cell transcripts is also observed in splenic cells activated with the T-cell mitogen concanavalin A (Con A). CD3-delta, -zeta, TcR-alpha and -beta mRNA expression increases, whilst CD3-gamma and -epsilon mRNA levels decrease after mitogenic activation. The potential implications of this regulation on the composition and expression of the TcR/CD3 complex during T-cell ontogeny and activation is discussed.
CD3蛋白转导由T细胞受体（TcR）传递的抗原信号。编码各个CD3亚基的基因在体外肿瘤细胞系中受到异步调控。在本报告中，我们研究了正常小鼠胸腺细胞在体内发育过程中各个CD3和TcR基因的表达情况。我们发现，在受孕后第14天（p.c.），CD3-γ、-δ、-ε和ζ转录本均有表达，同时还有IL-2Rα和Thy-1 mRNA，且早于功能性TcR-α、-β、CD4和CD8转录本的表达。随着发育进程，各个CD3亚基呈现出独特的基因表达增加模式。在用T细胞促有丝分裂原刀豆球蛋白A（Con A）激活的脾细胞中也观察到这些T细胞转录本的独特调控。有丝分裂原激活后，CD3-δ、-ζ、TcR-α和-β mRNA表达增加，而CD3-γ和-ε mRNA水平下降。本文讨论了这种调控对T细胞发育和激活过程中TcR/CD3复合物的组成和表达的潜在影响。
