在细胞毒性试验中，脂多糖与肿瘤坏死因子-α 协同作用。
Lipopolysaccharide synergizes with tumour necrosis factor-alpha in cytotoxicity assays.
作者信息
Pfister H, Hennet T, Jungi T W
机构信息
Institute of Veterinary Virology, University of Berne, Switzerland.
出版信息
Immunology. 1992 Nov;77(3):473-6.
Lipopolysaccharide (LPS) from Escherichia coli was found to synergize with human recombinant tumour necrosis factor-alpha (TNF-alpha) in the lysis of L929 and WEHI 164 (clone 13) murine fibroblasts, two cell lines classically used in TNF-alpha bioassays. The effect was noted with TNF-alpha at low (sublytic or lightly lytic) concentrations and was significant for LPS concentrations in the ng range. The LPS effect could be inhibited by polymyxin B, and was not observed when the TNF-alpha assay was performed in the absence of actinomycin D. Enhancement of TNF-alpha lysis by LPS occurred in several assays for determining TNF-alpha, including MTT cleavage, crystal violet staining and lactate dehydrogenase release. Synergism was obtained only when LPS and TNF-alpha were added to cells simultaneously, but not when applied in sequence. The reported synergism may be relevant for TNF-alpha determinations by bioassay, and for the understanding of pathophysiology of Gram-negative sepsis.
研究发现，来自大肠杆菌的脂多糖（LPS）能与人重组肿瘤坏死因子-α（TNF-α）协同作用，溶解L929和WEHI 164（克隆13）小鼠成纤维细胞，这两种细胞系是TNF-α生物测定中经典使用的细胞系。在低（亚溶解或轻度溶解）浓度的TNF-α时即可观察到这种效应，对于纳克范围内的LPS浓度而言该效应显著。LPS的作用可被多粘菌素B抑制，并且当在无放线菌素D的情况下进行TNF-α测定时未观察到该效应。在几种测定TNF-α的试验中，包括MTT裂解、结晶紫染色和乳酸脱氢酶释放试验，LPS均可增强TNF-α的溶解作用。仅当LPS和TNF-α同时添加到细胞中时才会产生协同作用，而按顺序添加则不会产生协同作用。所报道的这种协同作用可能与通过生物测定法测定TNF-α以及理解革兰氏阴性脓毒症的病理生理学有关。
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