采用高效液相色谱法测定血浆中的二甲双胍。

Determination of metformin in plasma by high-performance liquid chromatography.

作者信息

Huupponen R, Ojala-Karlsson P, Rouru J, Koulu M

机构信息

Department of Clinical Pharmacology, University of Turku, Finland.

出版信息

J Chromatogr. 1992 Dec 2;583(2):270-3. doi: 10.1016/0378-4347(92)80564-7.

DOI:10.1016/0378-4347(92)80564-7

PMID:1478993

Abstract

A high-performance liquid chromatographic method for the determination of metformin, an oral antidiabetic agent, in plasma is described. Plasma samples containing the internal standard, phenformin, are eluted through Amprep extraction columns before injection into the chromatographic column, packed with microBondapak phenyl. The eluent is monitored at 236 nm. At a mobile phase flow-rate of 1.35 ml/min, the retention times of metformin and phenformin are 2.8 and 5.6 min, respectively. The intra-day coefficients of variation are 1.5 and 4.3% at metformin concentrations of 0.05 and 1 mg/l, respectively.

摘要

本文描述了一种用于测定血浆中口服抗糖尿病药物二甲双胍的高效液相色谱法。含有内标物苯乙双胍的血浆样品在注入填充有微孔键合苯基的色谱柱之前，先通过Amprep萃取柱洗脱。洗脱液在236nm处进行监测。在流动相流速为1.35ml/min时，二甲双胍和苯乙双胍的保留时间分别为2.8分钟和5.6分钟。在二甲双胍浓度为0.05和1mg/l时，日内变异系数分别为1.5%和4.3%。