Albert M, Gallo J M, Escalier D, Parseghian N, Jouannet P, Schrevel J, David G
Laboratoire d'Embryologie, UFR Biomédicale des Saints Pères, Université Paris 5, France.
Hum Reprod. 1992 Oct;7(9):1249-56. doi: 10.1093/oxfordjournals.humrep.a137836.
To determine the acrosomal characteristics related to in-vitro fertilization (IVF) outcome, spermatozoa from 50 men whose wives had resorted to IVF have been studied by indirect immunofluorescence microscopy with anti-human pro-acrosin monoclonal antibody 4D4 (mAb 4D4), prior to and after incubation in a capacitating medium. The antibody labelled only the acrosomal principal region (APR), revealing its shape (i.e. normal, small or amorphous) and its status (i.e. unreacted, partially or totally reacted). The IVF outcome distinguished: (i) spermatozoa which were able to fertilize at least one oocyte in vitro (group I; n = 25) and (ii) spermatozoa which failed to fertilize any oocyte in vitro (group II; n = 25). The semen characteristics of the two sperm groups, including the acrosome morphology, were similar according to conventional analysis. The mAb 4D4 detected in both the whole and the swim-up sperm cell fractions a lower percentage of normal APR in group II (< 50% for 10 patients in group II versus one patient from group I), which was related to a higher percentage of small APR. Moreover, after 21 h incubation, group II had a lower acrosomal loss index. The spermatozoa of five patients of this infertile group II did not undergo acrosomal modification whereas spermatozoa of all group I patients underwent the acrosomal reaction. The data showed that the relationship between acrosomal anomalies and IVF failure is mainly due to an increased incidence of acrosomes with a reduced size of the region involved in the acrosome reaction. Immunodiagnosis of this acrosomal region by means of mAb 4D4 is informative for IVF outcome.
为了确定与体外受精（IVF）结果相关的顶体特征，我们使用抗人原顶体蛋白酶单克隆抗体4D4（mAb 4D4），通过间接免疫荧光显微镜对50名其妻子采用IVF的男性的精子进行了研究，研究在获能培养基中孵育前后进行。该抗体仅标记顶体主区域（APR），揭示其形状（即正常、小或无定形）及其状态（即未反应、部分或完全反应）。IVF结果分为：（i）能够在体外使至少一个卵母细胞受精的精子（第一组；n = 25）和（ii）在体外未能使任何卵母细胞受精的精子（第二组；n = 25）。根据传统分析，两组精子的精液特征，包括顶体形态，是相似的。mAb 4D4在整个精子细胞部分和上浮精子细胞部分中均检测到，第二组中正常APR的百分比更低（第二组10名患者中<50%，而第一组为一名患者），这与小APR的更高百分比相关。此外，孵育21小时后，第二组的顶体损失指数更低。该不育第二组的五名患者的精子未发生顶体修饰，而所有第一组患者的精子都发生了顶体反应。数据表明，顶体异常与IVF失败之间的关系主要是由于参与顶体反应的区域尺寸减小的顶体发生率增加。通过mAb 4D4对该顶体区域进行免疫诊断对IVF结果具有参考价值。
