Lecompte F, Combarnous Y
Station de Physiologie de la Reproduction des Mammifères Domestiques (PRMD), URA INRA/CNRS, Nouzilly, France.
J Immunoassay. 1992;13(4):483-93. doi: 10.1080/15321819208019830.
A simple, accurate, sensitive enzyme immunoassay (EIA) has been developed that permits the measurement of equine Chorionic Gonadotropin activity in pregnant mare plasmas or serums as well as in commercial and highly-purified preparations. This assay is specific for eCG and eLH which share the same polypeptide structure but differ in their oligosaccharidic chains. The more important result is that this EIA has been found to be give data in very close agreement with the in vivo assay. Therefore this very rapid and convenient assay can be used to measure the activity of eCG/PMSG in pregnant mares serums in in-field conditions as well as in crude or highly-purified preparations.
已开发出一种简单、准确、灵敏的酶免疫测定法（EIA），可用于测定孕马血浆或血清以及商业和高纯度制剂中的马绒毛膜促性腺激素活性。该测定法对具有相同多肽结构但寡糖链不同的eCG和eLH具有特异性。更重要的是，已发现这种EIA所给出的数据与体内测定法的数据非常吻合。因此，这种非常快速且方便的测定法可用于在现场条件下测定孕马血清中eCG/PMSG的活性，以及粗制或高纯度制剂中的活性。
