Resende M R, Silva M E, Silva M E, Silva M E, Bambirra E A, Vieira E C, Nicoli J R
Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
J Med. 1992;23(3-4):245-51.
The complete life cycle of Trypanosoma cruzi under germfree (GF) conditions was achieved. The Reduvidae insects Dipetalogaster maximus, reared under axenic conditions were infected with Trypanosoma cruzi, strain CL, by feeding on GF infected mice. Axenic trypomastigotes from macerated gnotobiotic insects were used to infect GF and conventional (CV) mice by intraperitoneal, ocular, and oral routes. Infection, followed by blood parasitemia, was obtained in almost all the cases. However, Chagas' disease was more severe in GF animals when compared with CV mice.
克氏锥虫在无菌（GF）条件下的完整生命周期已达成。在无菌条件下饲养的猎蝽科昆虫大腹圆蝽，通过取食GF感染小鼠而感染克氏锥虫CL株。将来自无菌昆虫匀浆的无菌锥鞭毛体通过腹腔、眼部和口服途径用于感染GF和常规（CV）小鼠。几乎在所有情况下都获得了感染及随后的血寄生虫血症。然而，与CV小鼠相比，GF动物的恰加斯病更为严重。
