Hanisch E, Herrmann G, Lemperle S, Jakobi R, Weber T, Wagner M
Zentrum der Chirurgie, Johann-Wolfgang-Goethe-Universität, Frankfurt/Main.
Langenbecks Arch Chir. 1992;377(6):360-7. doi: 10.1007/BF00574775.
Successful orthotopic liver transplantation (OLT) can be achieved in the rat. We used bromo-2-deoxyuridine (BrdU) as a proliferation marker to document morphological differences between OLT with and without rearterialization. Animals with portal anastomosis alone had a significantly increased proliferation rate of hepatocytes, Kupffer cells, and bile duct epithelial cells, as indicated by strong staining with BrdU, 8 days post-transplant compared to animals with rearterialization. Regeneration of ischemically damaged liver parenchymal cells may account for this observation. Thus, OLT with rearterialization appears to be the more physiological transplant model.
