赋予限制酶新的特异性：通过组合衔接子寡脱氧核苷酸和IIS类酶在任何预定位点进行切割。 - Suppr

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超能文献

Conferring new specificities on restriction enzymes: cleavage at any predetermined site by combining adapter oligodeoxynucleotide and class-IIS enzyme.

作者信息

Podhajska A J, Kim S C, Szybalski W

机构信息

Department of Microbiology, University of Gdansk, Poland.

出版信息

Methods Enzymol. 1992;216:303-9. doi: 10.1016/0076-6879(92)16028-i.

DOI:10.1016/0076-6879(92)16028-i

PMID:1479906

Abstract

摘要

相似文献

Conferring new specificities on restriction enzymes: cleavage at any predetermined site by combining adapter oligodeoxynucleotide and class-IIS enzyme.赋予限制酶新的特异性：通过组合衔接子寡脱氧核苷酸和IIS类酶在任何预定位点进行切割。

Methods Enzymol. 1992;216:303-9. doi: 10.1016/0076-6879(92)16028-i.

Cleaving DNA at any predetermined site with adapter-primers and class-IIS restriction enzymes.使用衔接子引物和IIS类限制性内切酶在任何预定位点切割DNA。

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XcmI as a universal restriction enzyme for single-stranded DNA.

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Universal restriction endonucleases: designing novel cleavage specificities by combining adapter oligodeoxynucleotide and enzyme moieties.通用限制性核酸内切酶：通过组合衔接子寡脱氧核苷酸和酶部分来设计新型切割特异性

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Class-IIS restriction enzymes--a review.IIS类限制酶——综述

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Conversion of the FokI endonuclease to a universal restriction enzyme: cleavage of phage M13mp7 DNA at predetermined sites.福克I型核酸内切酶转化为通用限制酶：在预定位点切割噬菌体M13mp7 DNA

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Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. V. Study of single-strand cleavages.EcoRII限制酶与修饰酶与合成DNA片段的相互作用。V. 单链切割研究。

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Assay of restriction endonucleases using oligonucleotides.使用寡核苷酸进行限制性内切酶分析。

Methods Mol Biol. 2001;148:465-90. doi: 10.1385/1-59259-208-2:465.

Alteration of the cleavage distance of Fok I restriction endonuclease by insertion mutagenesis.通过插入诱变改变福克I型限制性内切酶的切割距离。

Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2764-8. doi: 10.1073/pnas.90.7.2764.

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BMC Biochem. 2011 Dec 5;12:62. doi: 10.1186/1471-2091-12-62.

Restriction endonuclease-catalyzed cleavage of DNA stimulates subsequent polymerase chain reaction and enables discrimination between normal and mutant K-RAS alleles.

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Selective 'stencil'-aided pre-PCR cleavage of wild-type sequences as a novel approach to detection of mutant K-RAS.选择性“模板”辅助野生型序列的PCR前切割作为检测突变型K-RAS的新方法。

Nucleic Acids Res. 2001 Sep 1;29(17):E90-0. doi: 10.1093/nar/29.17.e90.

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