赋予限制酶新的特异性：通过组合衔接子寡脱氧核苷酸和IIS类酶在任何预定位点进行切割。 - Suppr

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超能文献

Conferring new specificities on restriction enzymes: cleavage at any predetermined site by combining adapter oligodeoxynucleotide and class-IIS enzyme.

作者信息

Podhajska A J, Kim S C, Szybalski W

机构信息

Department of Microbiology, University of Gdansk, Poland.

出版信息

Methods Enzymol. 1992;216:303-9. doi: 10.1016/0076-6879(92)16028-i.

DOI:10.1016/0076-6879(92)16028-i

PMID:1479906

Abstract

摘要

相似文献

Conferring new specificities on restriction enzymes: cleavage at any predetermined site by combining adapter oligodeoxynucleotide and class-IIS enzyme.赋予限制酶新的特异性：通过组合衔接子寡脱氧核苷酸和IIS类酶在任何预定位点进行切割。

Methods Enzymol. 1992;216:303-9. doi: 10.1016/0076-6879(92)16028-i.

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Class-IIS restriction enzymes--a review.IIS类限制酶——综述

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Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. V. Study of single-strand cleavages.EcoRII限制酶与修饰酶与合成DNA片段的相互作用。V. 单链切割研究。

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Assay of restriction endonucleases using oligonucleotides.使用寡核苷酸进行限制性内切酶分析。

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Alteration of the cleavage distance of Fok I restriction endonuclease by insertion mutagenesis.通过插入诱变改变福克I型限制性内切酶的切割距离。

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Restriction endonuclease-catalyzed cleavage of DNA stimulates subsequent polymerase chain reaction and enables discrimination between normal and mutant K-RAS alleles.

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