将心肌细胞与油酸和极低密度脂蛋白进行长期孵育会降低肝素可释放的脂蛋白脂肪酶活性。

Long term incubation of cardiac myocytes with oleic acid and very-low density lipoprotein reduces heparin-releasable lipoprotein lipase activity.

作者信息

Rodrigues B, Spooner M R, Severson D L

机构信息

Department of Pharmacology & Therapeutics, Faculty of Medicine, University of Calgary, Alberta, Canada.

出版信息

Mol Cell Biochem. 1992 Oct 21;116(1-2):33-7. doi: 10.1007/BF01270566.

DOI:10.1007/BF01270566

PMID:1480152

Abstract

An exogenous [3H]triolein emulsion was hydrolyzed by intact cardiac myocytes with functional LPL located on the cell surface. This surface-bound LPL could be released into the medium when cardiac myocytes were incubated with heparin. Incubation of cardiac myocytes with VLDL, or the products of TG breakdown, oleic acid or 2-monoolein, did not increase LPL activity in the medium. However, incubation of cardiac myocytes with either VLDL or oleic acid for > 60 min did reduce heparin-releasable LPL activity. In the heart, this inhibitory effect of FFA could regulate the translocation of LPL from its site of synthesis in the cardiac myocyte to its functional site at the capillary endothelium.

摘要

外源性[3H]三油酸甘油酯乳剂可被细胞表面具有功能性脂蛋白脂肪酶（LPL）的完整心肌细胞水解。当心肌细胞与肝素一起孵育时，这种表面结合的LPL可释放到培养基中。心肌细胞与极低密度脂蛋白（VLDL）或甘油三酯（TG）分解产物油酸或甘油单油酸酯一起孵育，并不会增加培养基中的LPL活性。然而，心肌细胞与VLDL或油酸孵育超过60分钟确实会降低肝素可释放的LPL活性。在心脏中，游离脂肪酸（FFA）的这种抑制作用可调节LPL从心肌细胞中的合成部位向毛细血管内皮细胞功能部位的转运。